血管生成素2(ANGPT2)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Angiopoietin 2 (ANGPT2)
ANG2; AGPT2
- 编号KSA009Bo01
- 物种Bos taurus; Bovine (Cattle,牛) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围12.5-800pg/mL
- 可适灵敏度5.4pg/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 8161 ¥ 13602 ¥ 24484 ¥ 47607 ¥ 81612
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对血管生成素2(ANGPT2)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Bos taurus; Bovine (Cattle,牛) | 应用(仅供研究使用,不用于临床诊断!) |
RPA009Bo01 | 血管生成素2(ANGPT2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA009Bo01 | 血管生成素2(ANGPT2)多克隆抗体 | WB; IHC; ICC; IP. |
KSA009Bo01 | 血管生成素2(ANGPT2)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
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Sci Rep. | Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. [pubmed:28276491] |
PeerJ | The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker [pubmed:28584715] |
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Scientific Reports | Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats [Pubmed: 32616735] |
Intensive Care Med Exp | The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats [33997943] |
Gynecol Endocrinol | Potential of thrombospondin-1 in treatment of polycystic ovary syndrome rat model: a preliminary study [34282706] |
Clinical and Experimental Immunology | Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients [Pubmed:35348620] |
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