载脂蛋白A1(APOA1)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Apolipoprotein A1 (APOA1)
Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I
- 编号KSA519Cp01
- 物种Capra hircus; Caprine (Goat,山羊) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围6.25-400ng/mL
- 可适灵敏度2.73ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7589 ¥ 12648 ¥ 22766 ¥ 44268 ¥ 75888
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对载脂蛋白A1(APOA1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Capra hircus; Caprine (Goat,山羊) | 应用(仅供研究使用,不用于临床诊断!) |
NPA519Cp01 | 载脂蛋白A1(APOA1)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA519Cp01 | 载脂蛋白A1(APOA1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA519Cp01 | 载脂蛋白A1(APOA1)多克隆抗体 | WB; IHC; ICC; IP. |
LAA519Cp71 | 载脂蛋白A1(APOA1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
KSA519Cp01 | 载脂蛋白A1(APOA1)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
European Journal of Applied Physiology | Endurance training enhances ABCA1 expression in rat small intestine [PubMed: 19629515] |
Official Journal of the INWR | Effect of eight weeks of wrestling and circuit fitness training on Apo lipoportein A-I and lymphocyte ABCA1 gene expression in well-trained wrestler. [Wrestling: source] |
Biochemical and Biophysical Research Communications | Treadmill exercise enhances ABCA1 expression in rat liver [ScienceDirect: S0006291X0701488X] |
World Journal of Sport Sciences | The Effect of Eight Weeks of Wrestling and Wrestling Technique Based Circuit Training on Lymphocyte ABCA1 Gene Expression and Plasma Apolipoprotein A-1 [Idosi: source] |
Biochimica et Biophysica Acta | Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties [PubMed: 23328279 ] |
Biomarkers | The association of a distinct plasma proteomic profile with the cervical high-grade squamous intraepithelial lesion of Uyghur women: a 2D liquid-phase chromatography/mass spectrometry study [PubMed: 22458349] |
Journal of the American Heart Association | MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE?/? Mice [PubMed: PMC3540673] |
PLoS ONE | Apolipoprotein AI Is a Potential Mediator of Remote Ischemic Preconditioning [Plosone: Source] |
Molecular Genetics and Metabolism | DYRK1A overexpression decreases plasma lecithin: cholesterol acyltransferase activity and apolipoprotein AI levels [Pubmed: 23920041] |
Endocrinology | Deficiency of Clusterin Exacerbates High-Fat Diet-Induced Insulin Resistance in Male Mice [Pubmed: 24684302] |
Molecular Genetics and Metabolism Reports | Corrective effects of hepatotoxicity by hepatic Dyrk1a gene delivery in mice with intermediate hyperhomocysteinemia [Sciencedirect:S2214426915000026] |
J Psychopharmacol | Anti-atherogenic properties of high-density lipoproteins in psychiatric patients before and after two months of atypical anti-psychotic therapy [PubMed: 26253619] |
Biochim Biophys Acta | Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253] |
Pharmacol Res Perspect | Efficacy of tomato concentrates in mouse models of dyslipidemia and cancer [PubMed: 26171234] |
Clin Exp Nephrol | Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome [PubMed: 26351173] |
Oncogene | Apolipoprotein A-I inhibits experimental colitis and colitis-propelled carcinogenesis [PubMed: 26279300] |
Oncogene | Apolipoprotein AI inhibits experimental colitis and colitis-propelled carcinogenesis [Pubmed:26279300] |
Allergy | Serum levels of 9α,11β-PGF2 and apolipoprotein A1 achieve high predictive power as biomarkers of anaphylaxis. [pubmed:28378321] |
Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
11 | High-Density Lipoproteins Exert Pro-inflammatory Effects on Macrophages via Passive Cholesterol Depletion and PKC-NF-kB/STAT1-IRF1 Signaling [pubmed:27866837] |
Biomedical Science Letters | Effect of Ginkgo biloba Extract (EGb 761) on Serum Cholesterol Levels in Wild-type C57Bl/6 Mice [10.15616/BSL.2017.23.2.80] |
ACS Biomaterials Science & Engineering | Enhanced Antiatherosclerotic Efficacy of Statin-Loaded Reconstituted High-Density Lipoprotein via Ganglioside GM1 Modification [abs:10.1021] |
Food Research International | Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo [Pubmed:29580541] |
Journal of The Science of Food and Agriculture | Consumption of orange fermented beverage improves antioxidant status and reduces peroxidation lipid and inflammatory markers in healthy humans [Pubmed:29124773] |
Toxicon | A membrane disrupting toxin from wasp venom underlies the molecular mechanism of tissue damage [Pubmed:29654869] |
European Journal of Pharmacology | The effect of exenatide (a GLP-1 analog) and sitagliptin (a DPP-4 inhibitor) on plasma platelet-activating factor acetylhydrolase (PAF-AH) activity and concentration in?… [Pubmed: 30768981] |
European Journal of Preventive Cardiology | High-intensity interval training has a greater effect on reverse cholesterol transport elements compared with moderate-intensity continuous training in obese male rats [Pubmed: 31718266] |
Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848] |
VETERINARY PATHOLOGY | Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats [Pubmed: 32880234] |
Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
Cardiovasc Toxicol | Interplay of Obesity, Ethanol, and Contaminant Mixture on Clinical Profiles of Cardiovascular and Metabolic Diseases: Evidence from an Animal Study [Pubmed:35429258] |
Peptides | Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor [Pubmed:35660638] |
Journal of Functional Foods | Castanea mollissima shell polyphenols regulate JAK2 and PPARγ expression to suppress inflammation and lipid accumulation by inhibiting M1 macrophages … [] |
留言咨询