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结缔组织生长因子(CTGF)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Connective Tissue Growth Factor (CTGF)

CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2

  • 结缔组织生长因子(CTGF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 结缔组织生长因子(CTGF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对结缔组织生长因子(CTGF)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Oryctolagus cuniculus (Rabbit，兔) 应用(仅供研究使用，不用于临床诊断！)
RPA010Rb01 结缔组织生长因子(CTGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA010Rb01 结缔组织生长因子(CTGF)多克隆抗体 WB; IHC; ICC; IP.
PAA010Rb51 结缔组织生长因子(CTGF)多克隆抗体 WB; IHC; ICC; IP.
LAA010Rb71 结缔组织生长因子(CTGF)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA010Rb21 结缔组织生长因子(CTGF)单克隆抗体 WB; IHC; ICC; IP.
SEA010Rb 结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA010Rb 结缔组织生长因子(CTGF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA010Rb 结缔组织生长因子(CTGF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
PSA010Rb01 结缔组织生长因子(CTGF)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA010Rb01 结缔组织生长因子(CTGF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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PLos One Activation of JNK Signaling Mediates Connective Tissue Growth Factor Expression and Scar Formation in Corneal Wound Healing [Plosone: 0032128]
Clinical Biochemistry Clinical significance of inflammatory and fibrogenic cytokines in diabetic nephropathy [ScienceDirect: S0009912012000987]
The Journal of Heart and Lung Transplantation Connective tissue growth factor (CTGF/CCN2): A protagonist in cardiac allograft vasculopathy development? [PubMed: S1053249812010121]
Biomedical and Environmental Sciences The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS Rats [Besjournal: Source]
Disease Markers Concentrations of connective tissue growth factor in patients with nonalcoholic fatty liver disease: Association with liver fibrosis [PubMed: 22846210]
World Journal of Gastroenterology Clinical significance of connective tissue growth factor in hepatitis B virus-induced hepatic fibrosis [PubMed: PMC3351780]
Journal of ethnopharmacology Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. [Pubmed: 24211397]
Radiation Oncology Cytokine levels as biomarkers of radiation fibrosis in patients treated with breast radiotherapy [Ro-journal: Source]
PLoS One. Knock Out of S1P3 Receptor Signaling Attenuates Inflammation and Fibrosis in Bleomycin-Induced Lung Injury Mice Model [Pubmed:Pmc4157792]
European Journal of Heart Failure Connective tissue growth factor and cardiac diastolic dysfunction: human data from the Taiwan Diastolic Heart Failure Registry and molecular basis by cellular and animal models [Pubmed:24464932]
Bone Connective Tissue Growth Factor is a Target of Notch Signaling in Cells of the Osteoblastic Lineage [Pubmed:24792956]
American Journal of Pathology Disruption of collagen homeostasis can reverse established age-related myocardial fibrosis [Pubmed:25701883]
J Gastroenterol Beneficial effects of combined ursodeoxycholic acid and angiotensin-II type 1 receptor blocker on hepatic fibrogenesis in a rat model of nonalcoholic steatohepatitis [PubMed: 26190501]
Life Sciences Suppression of connective tissue growth factor mediates the renoprotective effect of Sitagliptin rather than Pioglitazone in type 2 diabetes mellitus [Pubmed:27049870]
International Journal of Molecular Sciences TGF-β and Physiological Root Resorption of Deciduous Teeth. [pubmed:28035998]
Inflammation Research Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy [pubmed:27516211]
Heart & Vessels Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [pubmed:28004175]
British Journal of Ophthalmology The relationship between anti-vascular endothelial growth factor and fibrosis in proliferative retinopathy: clinical and laboratory evidence [pubmed:27531356]
Pathophysiology Molecular mechanisms underlying fibrosis and elastin destruction in childhood interstitial lung diseases [pubmed:27686729]
Evidence-Based Complementary and Alternative Medicine Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathway [pubmed:28698735]
Heart and Vessels Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [10.1007/s00380-016-0930-y]
Cytokine 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis. [pubmed:28460256]
Advanced Healthcare Materials Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. [pubmed:29120858]
Life Sciences Sustained β-AR stimulation induces synthesis and secretion of growth factors in cardiac myocytes that affect on cardiac fibroblast activation [Pubmed:29107793]
International Journal of Psychophysiology  Substrate Stiffness Influences the Time Dependence of CTGF Protein Expression in Müller Cells [Pubmed:30123889]
Journal of Cellular Physiology TGFβ mediates collagen production in human CRSsNP nasal mucosa‐derived fibroblasts through Smad2/3‐dependent pathway and CTGF induction and secretion [Pubmed: 30426494]
Journal of Clinical Medicine Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with … [Pubmed: 30413105]
Nature Communications Macrophage hypoxia signaling regulates cardiac fibrosis via Oncostatin M [Pubmed: 31249305]
Cardiology Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart Failure [Pubmed: 31466059]
Stem Cells International CTGF Attenuates Tendon-Derived Stem/Progenitor Cell Aging [Pubmed: 31827530]
Pharmaceuticals Small-Dose Sunitinib Modulates p53, Bcl-2, STAT3, and ERK1/2 Pathways and Protects against Adenine-Induced Nephrotoxicity [Pubmed: 33212804]
BMC Veterinary Research Lysophosphatidic acid as a regulator of endometrial connective tissue growth factor and prostaglandin secretion during estrous cycle and endometrosis in the … [Pubmed: 32943074]
Revista Portuguesa de Cardiologia MicroRNAs and ventricular remodeling in aortic stenosis [Pubmed: 32682570]
Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction []
Exp Gerontol High carbohydrate high fat diet causes arterial hypertension and histological changes in the aortic wall in aged rats: The involvement of connective tissue growth … [34455071]
Research Square Hepatocyte-specific Smad4 deficiency alleviates liver fibrosis via the p38/p65 pathway []
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