纤连蛋白(FN)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Fibronectin (FN)
FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein
- 编号KSA037Rb01
- 物种Oryctolagus cuniculus (Rabbit,兔) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围1.56-100ng/mL
- 可适灵敏度0.69ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6617 ¥ 11028 ¥ 19850 ¥ 38598 ¥ 66168
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对纤连蛋白(FN)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Oryctolagus cuniculus (Rabbit,兔) | 应用(仅供研究使用,不用于临床诊断!) |
SEA037Rb | 纤连蛋白(FN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA037Rb | 纤连蛋白(FN)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA037Rb01 | 纤连蛋白(FN)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
American Journal of Physiology - Renal Physiology | Advanced oxidation protein products induce mesangial cell perturbation through PKC-dependent activation of NADPH oxidase [PubMed: 19019916] |
Stem Cell Research Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly [Pubmed: 24739658] |
Molecules. | Identification of compounds from the water soluble extract of Cinnamomum cassia barks and their inhibitory effects against high-glucose-induced mesangial cells. [Pubmed: 24013407] |
Journal of Ethnopharmacology | Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathy [ScienceDirect: S0378874114002414] |
Investigative Ophthalmology & Visual Science | Inhibition by Female Sex Hormones of Collagen Gel Contraction Mediated by Retinal Pigment Epithelial Cells [Pubmed: 24609629] |
PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592] |
Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747] |
Laryngoscope. | Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria [Pubmed:25545625] |
Biomedical Materials | In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study [Pubmed:25634132] |
Biomedical Materials | In vivo [PubMed: 25634132] |
American Journal of Medical and Biological Research | Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate onNF-κB/leptin-insulin Axisin Obese Subjects with Non-alcoholic Fatty Liver Disease [Ajmbr: 3] |
Food Chem. | Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528] |
journal of perinatal medicine | Maternal endothelial damage as a disorder shared by early preeclampsia, late preeclampsia and intrauterine growth restriction. [pubmed:27865093] |
Chinese Journal of Contemporary Neurology and Neurosurgery | Effect of panax notoginseng saponins on efficacy and hemorrhagic transformation of rt-PA intravenous thrombolysis in patients with acute ischemic stroke [Article_en:CJFDTotal-XDJB201611014] |
Scientific Reports | Serum fbronectin distinguishes the early stages of hepatocellular carcinoma [pubmed:28842594] |
Molecular Medicine Reports | Pigment epithelium‑derived factor protects human glomerular mesangial cells from diabetes via NOXO1‑iNOS suppression [10.3892/mmr.2017.7563] |
Cell Death & Disease | Tenomodulin is essential for prevention of adipocyte accumulation and fibrovascular scar formation during early tendon healing. [pubmed:29022912] |
Journal of Cellular Biochemistry | Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL‐6 and IL‐8 in pulmonary fibrosis in rabbits [Pubmed:29932250] |
Biomedicine & Pharmacotherapy | Corn silk (Zea mays L.), a source of natural antioxidants with α-amylase, α-glucosidase, advanced glycation and diabetic nephropathy inhibitory activities [Pubmed: 30530231] |
Advanced Healthcare Materials | Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts [Pubmed: 30838809] |
Inflammation | TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling [Pubmed: 31111299] |
EBioMedicine | Three kinds of corneal host cells contribute differently to corneal neovascularization [] |
Renal Failure | Effects of autophagy on macrophage adhesion and migration in diabetic nephropathy [Pubmed: 31352855] |
Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy [Pubmed: 31511328] |
Neurotherapeutics | Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair [Pubmed: 31758411] |
FASEB Journal | Molecular hydrogen regulates PTEN‐AKT‐mTOR signaling via ROS to alleviate peritoneal dialysis‐related peritoneal fibrosis [Pubmed: 31930571] |
Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109] |
International Journal of Women¡¯s Health and Reproduction Sciences | Are the Circulating Levels of Copeptin and Fibronectin Dysregulated in Preeclamptic South African Black Women? [] |
Advanced glycation end-products associate with podocytopathy in type II diabetic patients [] | |
IBRO Neuroscience Reports | Early mobilization in spinal cord injury promotes changes in microglial dynamics and recovery of motor function [Pubmed:35586775] |
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