半乳糖凝集素3(GAL3)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Galectin 3 (GAL3)
LGALS3; CBP35; GALBP; GALIG; MAC2; 35 kDa lectin; Carbohydrate-binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; Laminin-binding protein; Lectin L-29
- 编号KSA303Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.054ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
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特异性
试剂盒中的抗体对半乳糖凝集素3(GAL3)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPA303Hu01 | 半乳糖凝集素3(GAL3)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA303Hu61 | 半乳糖凝集素3(GAL3)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA303Hu01 | 半乳糖凝集素3(GAL3)活性蛋白 | Cell culture; Activity Assays. |
PAA303Hu01 | 半乳糖凝集素3(GAL3)多克隆抗体 | WB; IHC; IF |
LAA303Hu71 | 半乳糖凝集素3(GAL3)多克隆抗体(生物素标记) | WB; IHC; ICC. |
LAA303Hu81 | 半乳糖凝集素3(GAL3)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
MAA303Hu24 | 半乳糖凝集素3(GAL3)单克隆抗体 | WB; IHC; ICC; IP. |
MAA303Hu23 | 半乳糖凝集素3(GAL3)单克隆抗体 | WB; IHC; ICC; IP. |
MAA303Hu22 | 半乳糖凝集素3(GAL3)单克隆抗体 | WB; IHC |
MAA303Hu21 | 半乳糖凝集素3(GAL3)单克隆抗体 | WB; IHC; ICC; IP. |
FAA303Hu02 | 抗半乳糖凝集素3(GAL3)单克隆抗体 | Flow cytometry. |
SEA303Hu | 半乳糖凝集素3(GAL3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA303Hu | 半乳糖凝集素3(GAL3)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA303Hu | 半乳糖凝集素3(GAL3)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA303Hu01 | 半乳糖凝集素3(GAL3)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
Clinica Chimica Acta | Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population [Pubmed:25578395] |
Scientific Reports | Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension [PubMed: 25747153] |
Int J Mol Sci | The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension [PubMed: 25984599] |
Scientifi Reportcs | Galectin-3 level and the severity of cardiac diastolic dysfunction using cellular and animal models and clinical indices [PubMed: 26582585] |
Clin Chim Acta | Galectin-1 and galectin-4: Plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population [PubMed: 25578396] |
JOURNAL OF THE AMERICAN SOCIETY OF HYPERTENSION | Galectin-3 mediates the pulmonary arterial hypertension-induced right ventricular remodeling through interacting with NADPH oxidase 4. [pubmed:28431936] |
昆明医科大学学报 | 半乳糖凝集素3 在COPD 和肺癌中的表达及其意义. [2095610X] |
Archives of Medical Science | Can we define migraine patients with blood high-sensitivity C-reactive protein and galectin-3 levels in the emergency department? [publication:305625095] |
Biosensors & Bioelectronics | A sensitive sandwich-type immunosensor for the detection of galectin-3 based on N-GNRs-Fe-MOFs@AuNPs nanocomposites and a novel AuPt-methylene blue nanorod. [pubmed:29096363] |
Scientific Reports | The profile of selected single nucleotide polymorphisms in patients with hypertension and heart failure with preserved and mid-range ejection fraction. [pubmed:28827564] |
Clinica Chimica Acta | Galectin-3 in patients with coronary heart disease and atrial fibrillation [Pubmed:29287900] |
Advances in Experimental Medicine and Biology | Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease [Pubmed:29429028] |
FASEB Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922] |
american journal of physiology-heart and circulatory physiology | Macrophage galectin-3 enhances intimal translocation of vascular calcification in diabetes mellitus [Pubmed: 32216615] |
Cardiology | Galectin-3 Induces Atrial Fibrosis by Activating the TGF-β1/Smad Pathway in Patients with Atrial Fibrillation [Pubmed: 32516780] |
AUSTRALIAN DENTAL JOURNAL | The effect of initial periodontal treatment on gingival crevicular fluid galectin©\3 levels in participants with periodontal disease [33378559] |
Cell Death & Disease | Trajectory modeling of endothelial-to-mesenchymal transition reveals galectin-3 as a mediator in pulmonary fibrosis [33771973] |
Int J Clin Pract | Serum Galectin©\3 Levels in Patients with Psoriasis [34137138] |
Phytomedicine | Zerumbone, a humulane sesquiterpene from Syringa pinnatifolia, attenuates cardiac fibrosis by inhibiting of the TGF-β1/Smad signaling pathway after myocardial … [Pubmed:35405613] |
Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive … [Pubmed:35687771] |
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