颗粒酶A(GZMA)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Granzyme A (GZMA)
CTLA3; HFSP; HF; H factor; Granzyme 1; Fragmentin-1; Hanukkah factor; Cytotoxic T-Lymphocyte-Associated Serine Esterase 3; CTL tryptase; Cytotoxic T-lymphocyte proteinase 1
- 编号KSA599Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.060ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对颗粒酶A(GZMA)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
EPA599Hu51 | 颗粒酶A(GZMA)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA599Hu01 | 颗粒酶A(GZMA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA599Hu06 | 颗粒酶A(GZMA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA599Hu51 | 颗粒酶A(GZMA)活性蛋白 | Cell culture; Activity Assays. |
EPA599Hu61 | 颗粒酶A(GZMA)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA599Hu07 | 颗粒酶A(GZMA)多克隆抗体 | WB; IHC; ICC; IP. |
PAA599Hu51 | 颗粒酶A(GZMA)多克隆抗体 | WB; IHC; ICC; IP. |
PAA599Hu01 | 颗粒酶A(GZMA)多克隆抗体 | WB; IHC; ICC; IP. |
PAA599Hu06 | 颗粒酶A(GZMA)多克隆抗体 | WB; IHC; ICC; IP. |
SEA599Hu | 颗粒酶A(GZMA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEA599Hu | 颗粒酶A(GZMA)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA599Hu | 颗粒酶A(GZMA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA599Hu01 | 颗粒酶A(GZMA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Cell Reports | Granzyme A Produces Bioactive IL-1β through a Nonapoptotic Inflammasome-Independent Pathway [Pubmed:25437548] |
Journal of Dermatological Science | Relationships among plasma granzyme B level, pruritus and dermatitis in patients with atopicdermatitis. [pubmed:27686401] |
Journal of Maternal-Fetal & Neonatal Medicine | Extracellular granzyme A in amniotic fluid is elevated in the presence of sterile intra-amniotic inflammation in preterm prelabor rupture of membranes [Pubmed: 32912008] |
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