生长分化因子5(GDF5)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Growth Differentiation Factor 5 (GDF5)
CDMP1; LAP4; SYNS2; BMP14; Radotermin; Cartilage-Derived Morphogenetic Protein-1; Bone morphogenetic protein 14; Lipopolysaccharide-associated protein 4
- 编号KSC110Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围78-5000pg/mL
- 可适灵敏度32pg/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6048 ¥ 10080 ¥ 18144 ¥ 35280 ¥ 60480
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特异性
试剂盒中的抗体对生长分化因子5(GDF5)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPC110Hu01 | 生长分化因子5(GDF5)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC110Hu02 | 生长分化因子5(GDF5)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC110Hu01 | 生长分化因子5(GDF5)多克隆抗体 | WB; IHC; ICC; IP. |
PAC110Hu02 | 生长分化因子5(GDF5)多克隆抗体 | WB; IHC; ICC; IP. |
LAC110Hu81 | 生长分化因子5(GDF5)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAC110Hu71 | 生长分化因子5(GDF5)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAC110Hu22 | 生长分化因子5(GDF5)单克隆抗体 | WB; IHC; ICC; IP. |
SEC110Hu | 生长分化因子5(GDF5)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
IEC110Hu | 生长分化因子5(GDF5)检测试剂盒(酶联免疫吸附试验法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCC110Hu | 生长分化因子5(GDF5)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMC110Hu | 生长分化因子5(GDF5)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSC110Hu01 | 生长分化因子5(GDF5)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
Carbohydrate Polymers | ZrO2 surface chemically coated with hyaluronic acid hydrogel loading GDF-5 for osteogenesis in dentistry [PubMed: 23218279] |
Bone | Photo-cured hyaluronic acid-based hydrogels containing growth and differentiation factor 5 (GDF-5) for bone tissue regeneration [ScienceDirect: S875632821300481X] |
J Nanosci Nanotechnol | In Vitro Osteogenic Differentiation Enhanced by Zirconia Coated with Nano-Layered Growth and Differentiation Factor-5 [Pubmed:27398455] |
Macromolecular Research | Osteoblastic Differentiation of Functionalized Biphasic Hydroxyapatite and b-Tricalcium Phosphate with Recombinant Human Growth and Differentiation (rhGDF-5) [] |
Pharmaceutics | 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells [34575523] |
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