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27kDa热休克蛋白(Hsp27)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Heat Shock Protein 27 (Hsp27)

HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;

  • 27kDa热休克蛋白(Hsp27)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 27kDa热休克蛋白(Hsp27)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对27kDa热休克蛋白(HSPB1)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
EPA693Hu61 27kDa热休克蛋白(Hsp27)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA693Hu01 27kDa热休克蛋白(Hsp27)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA693Hu61 27kDa热休克蛋白(Hsp27)活性蛋白 Cell culture; Activity Assays.
PAA693Hu01 27kDa热休克蛋白(Hsp27)多克隆抗体 WB,IHC,ICC/IF
FAA693Hu01 抗27kDa热休克蛋白(Hsp27)多克隆抗体 FCM
LAA693Hu81 27kDa热休克蛋白(Hsp27)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA693Hu71 27kDa热休克蛋白(Hsp27)多克隆抗体(生物素标记) WB; IHC; ICC.
FAA693Hu81 抗27kDa热休克蛋白(Hsp27)多克隆抗体(异硫氰酸荧光素标记) Flow cytometry.
MAA693Hu22 27kDa热休克蛋白(Hsp27)单克隆抗体 WB; IHC; ICC; IP.
MAA693Hu21 27kDa热休克蛋白(Hsp27)单克隆抗体 WB; IHC; ICC; IP.
MAA693Hu23 27kDa热休克蛋白(Hsp27)单克隆抗体 WB; IHC
MAA693Hu24 27kDa热休克蛋白(Hsp27)单克隆抗体 WB; IHC; ICC; IP.
MAA693Hu25 27kDa热休克蛋白(Hsp27)单克隆抗体 WB; IHC; ICC; IP.
MAA693Hu27 27kDa热休克蛋白(Hsp27)单克隆抗体 WB; IHC; ICC; IP.
MAA693Hu29 27kDa热休克蛋白(Hsp27)单克隆抗体 WB; IHC; ICC; IP.
FAA693Hu06 抗27kDa热休克蛋白(Hsp27)单克隆抗体 Flow cytometry.
FAA693Hu08 抗27kDa热休克蛋白(Hsp27)单克隆抗体 Flow cytometry.
FAA693Hu04 抗27kDa热休克蛋白(Hsp27)单克隆抗体 Flow cytometry.
FAA693Hu02 抗27kDa热休克蛋白(Hsp27)单克隆抗体 Flow cytometry.
LAA693Hu74 27kDa热休克蛋白(Hsp27)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA693Hu 27kDa热休克蛋白(Hsp27)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA693Hu 27kDa热休克蛋白(Hsp27)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA693Hu 27kDa热休克蛋白(Hsp27)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA693Hu01 27kDa热休克蛋白(Hsp27)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Animal Science Journal The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells [PubMed: 26467738]
Nature Communications HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis [Pubmed:29650953]
Advances in Experimental Medicine and Biology Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease [Pubmed:29429028]
Diabetes Research and Clinical Practice The beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacity [Pubmed: 31102684]
Journal of Immunology Research Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients []
Journal of Clinical Medicine Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109]
Medicine Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive … [Pubmed:35687771]
Beilstein Archives Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation []
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