血红素氧合酶1(HO1)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Heme Oxygenase 1 (HO1)
HMOX1; Hsp32; HMOX1D
- 编号KSA584Po01
- 物种Sus scrofa; Porcine (Pig,猪) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.312-20ng/mL
- 可适灵敏度0.119ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7607 ¥ 12678 ¥ 22820 ¥ 44373 ¥ 76068
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对血红素氧合酶1(HO1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Sus scrofa; Porcine (Pig,猪) | 应用(仅供研究使用,不用于临床诊断!) |
RPA584Po01 | 血红素氧合酶1(HO1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA584Po02 | 血红素氧合酶1(HO1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA584Po01 | 血红素氧合酶1(HO1)多克隆抗体 | WB; IHC; ICC; IP. |
PAA584Po02 | 血红素氧合酶1(HO1)多克隆抗体 | WB; IHC; ICC; IP. |
LAA584Po81 | 血红素氧合酶1(HO1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAA584Po71 | 血红素氧合酶1(HO1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
KSA584Po01 | 血红素氧合酶1(HO1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
The American Journal of Pathology | Characterization of the Intrarenal Renin-Angiotensin System in Experimental Alport Syndrome [PubMed: 25777063] |
BioMed Research International | Serum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing Spondylitis [Pubmed:27314037] |
Cancer Medicine | Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease [Pubmed:27109254] |
Redox Report: Communications in Free Radical Research | Decreased expression of heme oxygenase is associated with depressive symptoms and may contribute to depressive and hypertensive comorbidity [Pubmed:26824276] |
Canadian Journal of Physiology and Pharmacology | Sulforaphane protects against sodium valproate–induced acute liver injury [pubmed:28112972] |
Peptides. | Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms. [pubmed:27777064] |
european journal of pharmacology | S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase. [pubmed:27887948] |
brain research | Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling. [pubmed:28238669] |
biomedicine & pharmacotherapy | Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats. [pubmed:28068636] |
Inflammopharmacology. | Implication of Nrf2/HO-1 pathway in the coloprotective effect of coenzyme Q10 against experimentally induced ulcerative colitis. [pubmed:28050757] |
Environmental Toxicology and Pharmacology | Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study [pubmed:28192751] |
Journal of Microbiology, Immunology and Infection | Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients [pubmed:28655573] |
Nutrition & Metabolism | Hepatic, adipocyte, enteric and pancreatic hormones: response to dietary macronutrient composition and relationship with metabolism [pubmed:28694840] |
Biomedicine & Pharmacotherapy | Ameliorative potential of sitagliptin and/or resveratrol on experimentallyinduced clear cell renal cell carcinoma [pubmed:29101811] |
Acta Paediatrica | Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children [DOI: 10.1111/apa.14106] |
Naunyn-Schmiedeberg's Arch Pharmacol | Quercetin and tin protoporphyrin attenuate hepatic ischemia reperfusion injury: role of HO-1 [pubmed:28589248] |
Asian Pacific Journal of Cancer Prevention | Ameliorative Effect of Coenzyme Q10 and/or Candesartan on Carboplatin-Induced Nephrotoxicity: Roles of Apoptosis, Transforming Growth Factor-Β1, Nuclear Factor Kappa-B And The Nrf2/HO-1 Pathway [pubmed:28670881] |
Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms [pubmed:28061403] |
Talanta | Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment … [10.1016:j.talanta.2018.01.044] |
Pharmacological Reports | The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder [10.1016:j.pharep.2018.06.001] |
Physiology & Behavior | Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll … [Pubmed:29410018] |
Journal of Infection and Chemotherapy | Targeting the proinflammatory cytokines, oxidative stress, apoptosis and TGF-β1/STAT-3 signaling by irbesartan to ameliorate doxorubicin-induced hepatotoxicity [Pubmed:29753615] |
American Institute of Physics | Anti-hemolytic and anti-inflammatory activities of the methanolic extract of (P.Beauv.) Briq. leaves in 2-butoxyethanol-hemolytic induced … [10.1063:1.5033415] |
Iranian Journal of Basic Medical Sciences | Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress [Doi: 10.22038/IJBMS.2018.31716.7714] |
Biomedicine & Pharmacotherapy | Targeting Nrf2/HO-1 signaling by crocin: Role in attenuation of AA-induced ulcerative colitis in rats [Pubmed: 30530041] |
Experimental Parasitology | Sodium arsenite augments sensitivity of Echinococcus granulosus protoscoleces to albendazole [Pubmed: 30790573] |
JGH OPEN | Cilostazol against 2, 4, 6‐trinitrobenzene sulfonic acid‐induced colitis: Effect on tight junction, inflammation, and apoptosis [] |
Journal of Food Biochemistry | Vanillic acid alleviates palmitic acid‐induced oxidative stress in human umbilical vein endothelial cells via Adenosine Monophosphate‐Activated Protein Kinase … [] |
Experimental and Therapeutic Medicine | Effect of heme oxygenase 1 and renin/prorenin receptor on oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cells [] |
Life Sciences | Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis [Pubmed: 31465731] |
Respiratory physiology & neurobiology | Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosis [Pubmed: 31600583] |
Life Sci | The protective effect of Lactobacillus versus 5-aminosalicylic acid in ulcerative colitis model by modulation of gut microbiota and Nrf2/Ho-1 pathway [Pubmed: 32526285] |
JAMA Surgery | Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairment [Pubmed: 28593326] |
JOURNAL OF PHARMACY AND PHARMACOLOGY | Isoliquiritigenin attenuates inflammation and modulates Nrf2/caspase-3 signalling in STZ-induced aortic injury [33793806] |
Exp Parasitol | In vitro protoscolicidal effects of lithocholic acid on protoscoleces of Echinococcus granulosus and its mechanism [Pubmed:35594934] |
Free Radical Biology and Medicine | Flavonoid 4, 4′-dimethoxychalcone induced ferroptosis in cancer cells by synergistically activating Keap1/Nrf2/HMOX1 pathway and inhibiting FECH [Pubmed:35697292] |
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