硫酸肝素蛋白聚糖(HSPG)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Heparan Sulfate Proteoglycan (HSPG)
SDC2; HSPG1; SYND2; Fibroglycan; Syndecan 2; Syndecan Proteoglycan 2; Heparan Sulfate Proteoglycan 1,Cell Surface-Associated; Heparan Sulphate Proteoglycan
- 编号KSA565Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围15.6-1000pg/mL
- 可适灵敏度5.9pg/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对硫酸肝素蛋白聚糖(HSPG)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA565Hu01 | 硫酸肝素蛋白聚糖(HSPG)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA565Hu01 | 硫酸肝素蛋白聚糖(HSPG)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA565Hu71 | 硫酸肝素蛋白聚糖(HSPG)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA565Hu81 | 硫酸肝素蛋白聚糖(HSPG)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA565Hu22 | 硫酸肝素蛋白聚糖(HSPG)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA565Hu | 硫酸肝素蛋白聚糖(HSPG)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA565Hu | 硫酸肝素蛋白聚糖(HSPG)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA565Hu01 | 硫酸肝素蛋白聚糖(HSPG)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Journal of Applied Pharmaceutical Science | Cytotoxic effects of antiglypican-3 against HepG3 cell line [Ebscohost: Source] |
| Journal of Reproductive Immunology | Fibrinogen, an endogenous ligand of Toll-like receptor 4, activates monocytes in pre-eclamptic patients [Pubmed: 24661950] |
| European journal of pharmacology | Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme [Pubmed: 24530413] |
| European Journal of Pharmacology | Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma [Pubmed:25449037] |
| Shock | Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance [Pubmed:25692257] |
| Biomarkers in Medicine | Endothelial glycocalyx layer shedding following lung resection [pubmed:27643669] |
| Apmis | Circulating syndecans during critical illness. [pubmed:28256016] |
| Biomedicine & Pharmacotherapy | Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and [Pubmed:29669302] |
| Drug Delivery | Glioma targeting peptide modified apoferritin nanocage [Pubmed:29726297] |
| Anaesthesia | Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass [Pubmed: 30687934] |
| Animals | Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period … [] |
| LIFE SCIENCES | Heparin prevents in vitro glycocalyx shedding induced by plasma from COVID-19 patients [33781826] |
| Genet Mol Biol | A novel mutation in ext2 caused hereditary multiple exostoses through reducing the synthesis of heparan sulfate [34042151] |
| Poultry Science | Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine?¡ [33518110] |
| J Gerontol A Biol Sci Med Sci | The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo … [34423832] |
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