角蛋白18(CK18)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Cytokeratin 18 (CK18)
KRT18; CYK18; K18; Keratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18
- 编号KSB231Ra01
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.058ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
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特异性
试剂盒中的抗体对角蛋白18(KRT18)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPB231Ra01 | 角蛋白18(CK18)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB231Ra01 | 角蛋白18(CK18)多克隆抗体 | WB; IHC; ICC; IP. |
MAB231Ra21 | 角蛋白18(CK18)单克隆抗体 | WB; IHC; ICC; IP. |
SEB231Ra | 角蛋白18(CK18)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB231Ra | 角蛋白18(CK18)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB231Ra01 | 角蛋白18(CK18)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Can J Physiol Pharmacol. | Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis [Pubmed:27186801] |
J Alzheimers Dis | Cigarette Smoke-Induced Alterations in Frontal White Matter Lipid Profiles Demonstrated by MALDI-Imaging Mass Spectrometry: Relevance to Alzheimer's Disease [Pubmed:26836183] |
bioscience biotechnology research asia | Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring [] |
Biomed Chromatogr | Lipidomic‐based investigation into the therapeutic effects of PPC and BBD on rats with NAFLD [34727379] |
J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … [Pubmed:35212074] |
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