可罗索(KL)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Klotho (KL)
KLa; Klotho Alpha
- 编号KSH757Mu01
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围3.12-200pg/mL
- 可适灵敏度1.25pg/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7776 ¥ 12960 ¥ 23328 ¥ 45360 ¥ 77760
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对可罗索(KL)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPH757Mu02 | 可罗索(KL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPH757Mu01 | 可罗索(KL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH757Mu02 | 可罗索(KL)多克隆抗体 | WB; IHC; ICC; IP. |
| PAH757Mu01 | 可罗索(KL)多克隆抗体 | WB; IHC; ICC; IP. |
| LAH757Mu71 | 可罗索(KL)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAH757Mu81 | 可罗索(KL)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| SEH757Mu | 可罗索(KL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEH757Mu | 可罗索(KL)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCH757Mu | 可罗索(KL)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMH757Mu | 可罗索(KL)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSH757Mu01 | 可罗索(KL)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Life Sciences | High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculature [Pubmed: 24012609] |
| EXPERIMENTAL & CLINICAL CARDIOLOGY | Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in Mice [Com:Source] |
| Int J Clin Exp Pathol.? | α-Klotho is an acute phase protein and altered by restraint stress in mice [Pubmed:Pmc4203207] |
| Annales de Biologie Clinique | Les biomarqueurs des calcifications vasculaires [PubMed: 26069069] |
| Mol Med Rep | Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with Klotho [PubMed: 25873020] |
| Calcif Tissue Int | Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3 [PubMed: 25899975] |
| 中华内分泌代谢杂志 | 抗衰老蛋白Klotho 阻断高糖培养的大鼠肾小球系膜细胞TLR4/NF-κB p65/NGAL 通路的激活及其作用机制 [Cn: Article] |
| Mol Cell Endocrinol | The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanism [PubMed: 26419931] |
| Arch Med Sci | Soluble Klotho protein as a novel serum biomarker in patients with acromegaly [pmc:PMC4754356] |
| Am J Nephrol | Klotho Deficiency Aggravates Tacrolimus-Induced Renal Injury via the Phosphatidylinositol 3-Kinase-Akt-Forkhead Box Protein O Pathway [Pubmed:27174564] |
| Sci Rep. | Klotho preservation via histone deacetylase inhibition attenuates chronic kidney disease-associated bone injury in mice. [pubmed:28387374] |
| Nephrology | Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management? [pubmed:28429560] |
| Journal of Integrative Nephrology & Andrology | Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patients [issn:2394-2916] |
| International Journal of Clinical and Experimental Pathology | Protective effect of restructuring klotho protein combined with trimetazidine on myocardial injury in mice [ISSN:1936-2625] |
| Journal of veterinary medical science | Changes of Klotho protein and Klotho mRNA expression in a hydroxy-L-proline induced hyperoxaluric rat model [pubmed:28943597] |
| Cellular Physiology and Biochemistry | Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion Injury [Pubmed:29550818] |
| American Journal of Translational Research | Advanced maternal age impairs spatial learning capacity in young adult mouse offspring [Pubmed:29636887] |
| Journal of Diabetes and its Complications | Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complications [Pubmed:30042059] |
| Medical Science Monitor | Klotho Inhibits Proliferation and Migration of Angiotensin II-Induced Vascular Smooth Muscle Cells (VSMCs) by Modulating NF-κB p65, Akt, and Extracellular Signal … [Pubmed:30004089] |
| Nephrology Dialysis Transplantation | High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney disease [Pubmed: 30189026] |
| BMC Nephrology | The role of klotho in chronic kidney disease [Pubmed: 30348110] |
| Nature Communications | Age-related declines in α-Klotho drive progenitor cell mitochondrial dysfunction and impaired muscle regeneration [Pubmed: 30451844] |
| Kidney International | Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome [Pubmed: 30770217] |
| Expert Opinion on Therapeutic Targets | The use of LP533401 as a therapeutic option for renal osteodystrophy affects, renal calcium handling, vitamin D metabolism and bone health in uremic rats [Pubmed: 30801205] |
| Mol Immunol | CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection [Pubmed: 33223223] |
| Journal of Clinical Hypertension | Effects of salt intervention on serum levels of Klotho influenced by salt sensitivity [Pubmed: 33164306] |
| CURRENT EYE RESEARCH | Expression of α-Klotho is downregulated and associated with oxidative stress in the lens in streptozotocin-induced diabetic rats [Pubmed: 32744464] |
| international immunopharmacology | Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic rats [Pubmed: 32438076] |
| Purinergic Signalling | P2X7 siRNA targeted to the kidneys increases klotho and delays the progression of experimental diabetic nephropathy. [Pubmed: 32377919] |
| Oxidative Medicine and Cellular Longevity | Ameliorating Effect of Klotho Protein on Rat Heart during I/R Injury [Pubmed: 33123314] |
| NEUROSCIENCE | Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With Schizophrenia [Pubmed: 32612508] |
| Pharmacological Reports | ¦ÁKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy [33675964] |
| J Mol Neurosci | Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma Cells [Pubmed:35112226] |
| Reproductive Biology and Endocrinology | Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance [] |
| Journal of Ovarian Research | Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome [] |
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