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脂蛋白脂酶(LPL)检测试剂盒DIY材料(酶联免疫吸附试验法)

试剂盒中的抗体对脂蛋白脂酶(LIPD)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

杂志	参考文献
Diabetes	Severity of Diabetes Governs Vascular Lipoprotein Lipase by Affecting Enzyme Dimerization and Disassembly [PubMed: 21646389]
The Korean Journal of Internal Medicine	Clinical efficacy of serum lipase subtype analy-sis for the differential diagnosis of pancreatic and non-pancreatic lipase elevation [Pubmed:27243230]
The Journal of Lipid Research	ReCavia (Guinea pig )lation of Plasma Lipid Homeostasis by Hepatic Lipoprotein Lipase in Adult Mice [Pubmed:27234787]
Journal of Functional Foods	Polysaccharides from Cyclocarya paliurus: Chemical composition and lipid-lowering effect on rats challenged with high-fat diet [10.1016/j.jff.2017.07.020]
Biotechnology Progress	Mass Spectrometric Evaluation of Upstream and Downstream Process Influences on Host Cell Protein Patterns in Biopharmaceutical Products [Pubmed: 30767403]
arteriosclerosis thrombosis and vascular biology	Integrin β3 Deficiency Results in Hypertriglyceridemia Via Disrupting LPL (Lipoprotein Lipase) Secretion [Pubmed: 32237906]
Clinical Nutrition ESPEN	Abdominal fat distribution modulates the metabolic effects of exogenous ketones in individuals with new-onset prediabetes after acute pancreatitis: Results from a randomized placebo-controlled trial [34024503]
Antioxidants	Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory … [Pubmed:35204215]

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编号	适用物种：Rattus norvegicus (Rat，大鼠)	应用(仅供研究使用，不用于临床诊断！)
RPA386Ra01	脂蛋白脂酶(LPL)重组蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAA386Ra01	脂蛋白脂酶(LPL)多克隆抗体	WB; IHC; ICC; IP.
MAA386Ra21	脂蛋白脂酶(LPL)单克隆抗体	WB; IHC; ICC; IP.
SEA386Ra	脂蛋白脂酶(LPL)检测试剂盒(酶联免疫吸附试验法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA386Ra	脂蛋白脂酶(LPL)等多因子检测试剂盒(流式荧光发光法)	FLIA Kit for Antigen Detection.
KSA386Ra01	脂蛋白脂酶(LPL)检测试剂盒DIY材料(酶联免疫吸附试验法)	自己动手制作ELISA试剂盒的主要材料
PGA386Ra01	脂蛋白脂酶(LPL)引物对	PCR