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赖氨酰氧化酶(LOX)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Lysyl Oxidase (LOX)

Protein-Lysine 6-Oxidase

  • 赖氨酰氧化酶(LOX)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 赖氨酰氧化酶(LOX)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对赖氨酰氧化酶(LOX)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APC580Hu01 赖氨酰氧化酶(LOX)活性蛋白 Cell culture; Activity Assays.
RPC580Hu01 赖氨酰氧化酶(LOX)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC580Hu02 赖氨酰氧化酶(LOX)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPC580Hu61 赖氨酰氧化酶(LOX)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC580Hu02 赖氨酰氧化酶(LOX)多克隆抗体 WB; IHC; ICC; IP.
PAC580Hu01 赖氨酰氧化酶(LOX)多克隆抗体 WB; IHC; ICC; IP.
LAC580Hu81 赖氨酰氧化酶(LOX)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAC580Hu71 赖氨酰氧化酶(LOX)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC580Hu22 赖氨酰氧化酶(LOX)单克隆抗体 WB; IHC; ICC; IP.
SEC580Hu 赖氨酰氧化酶(LOX)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
WEC580Hu 赖氨酰氧化酶(LOX)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC580Hu 赖氨酰氧化酶(LOX)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSC580Hu01 赖氨酰氧化酶(LOX)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
American Journal of Hematology The expression of lysyl-oxidase gene family members in myeloproliferative neoplasms. [PubMed: 23494965]
Current Eye Research High Glucose Induced Differential Expression of Lysyl Oxidase and Its Isoform in ARPE-19 Cells [Pubmed: 22966818]
European Journal of Surgical Oncology Evaluation of serum lysyl oxidase as a blood test for colorectal cancer [Pubmed: 24246612]
Vaccine Copia autorizada por CDR [Ingentaconnect: Source]
Arthritis & Rheumatology Lysyl oxidase is a potential biomarker of fibrosis in systemic sclerosis [Pubmed: 24574233]
Eur J Surg Oncol.? Evaluation of serum lysyl oxidase as a blood test for colorectal cancer [Pubmed:Pmc4237001]
BMC Cancer. Ionizing radiation induces tumor cell lysyl oxidase secretion [Pubmed:Pmc4223762]
DNA Cell Biol. Lysyl Oxidase Genetic Variants Affect Gene Expression in Cervical Cancer [Pubmed:24945327]
scandinavian journal of immunology Increased Serum Levels of the IL-33 Neutralizing sST2 in Limited Cutaneous Systemic Sclerosis [PubMed: 26095613]
Curr Eye Res Correlation of Aqueous Humor Lysyl Oxidase Activity with TGF-ß Levels and LOXL1 Genotype in Pseudoexfoliation [Pubmed:27116380]
journal of bone and mineral metabolism LOX Fails to Substitute for RANKL in Osteoclastogenesis. [pubmed:27606829]
38 Blocking Surgically Induced Lysyl Oxidase Activity Reduces the Risk of Lung Metastases. [pubmed:28445728]
Molecular Medicine Reports Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritis [pubmed:28901438]
Revista Española de Cardiología (English Edition) A Global Assessment of Circulating Prolysyl Oxidase in Nonischemic Patients With Garden-variety Heart Failure With Preserved Ejection Fraction [Pubmed:29807761]
Molecular Medicine Reports Lysyl oxidase inhibition via β-aminoproprionitrile hampers human umbilical vein endothelial cell angiogenesis and migration in vitro [Pubmed:29393489]
Thyroid Lysyl Oxidase is a key player in BRAF/MAPK pathway-driven thyroid cancer aggressiveness [Pubmed: 30398411]
Am J Physiol Lung Cell Mol Physiol Lysyl Oxidase Directly Contributes to Extracellular Matrix Production and Fibrosis in Systemic Sclerosis. [Pubmed: 33026236]
J Am Heart Assoc Collagen Cross©\Linking Is Associated With Cardiac Remodeling in Hypertrophic Obstructive Cardiomyopathy [33356379]
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