赖氨酰氧化酶(LOX)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Lysyl Oxidase (LOX)
Protein-Lysine 6-Oxidase
- 编号KSC580Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.061ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7560 ¥ 12600 ¥ 22680 ¥ 44100 ¥ 75600
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对赖氨酰氧化酶(LOX)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
APC580Hu01 | 赖氨酰氧化酶(LOX)活性蛋白 | Cell culture; Activity Assays. |
RPC580Hu01 | 赖氨酰氧化酶(LOX)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC580Hu02 | 赖氨酰氧化酶(LOX)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPC580Hu61 | 赖氨酰氧化酶(LOX)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC580Hu02 | 赖氨酰氧化酶(LOX)多克隆抗体 | WB; IHC; ICC; IP. |
PAC580Hu01 | 赖氨酰氧化酶(LOX)多克隆抗体 | WB; IHC; ICC; IP. |
LAC580Hu81 | 赖氨酰氧化酶(LOX)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAC580Hu71 | 赖氨酰氧化酶(LOX)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAC580Hu22 | 赖氨酰氧化酶(LOX)单克隆抗体 | WB; IHC; ICC; IP. |
SEC580Hu | 赖氨酰氧化酶(LOX)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
WEC580Hu | 赖氨酰氧化酶(LOX)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC580Hu | 赖氨酰氧化酶(LOX)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSC580Hu01 | 赖氨酰氧化酶(LOX)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
American Journal of Hematology | The expression of lysyl-oxidase gene family members in myeloproliferative neoplasms. [PubMed: 23494965] |
Current Eye Research | High Glucose Induced Differential Expression of Lysyl Oxidase and Its Isoform in ARPE-19 Cells [Pubmed: 22966818] |
European Journal of Surgical Oncology | Evaluation of serum lysyl oxidase as a blood test for colorectal cancer [Pubmed: 24246612] |
Vaccine | Copia autorizada por CDR [Ingentaconnect: Source] |
Arthritis & Rheumatology | Lysyl oxidase is a potential biomarker of fibrosis in systemic sclerosis [Pubmed: 24574233] |
Eur J Surg Oncol.? | Evaluation of serum lysyl oxidase as a blood test for colorectal cancer [Pubmed:Pmc4237001] |
BMC Cancer. | Ionizing radiation induces tumor cell lysyl oxidase secretion [Pubmed:Pmc4223762] |
DNA Cell Biol. | Lysyl Oxidase Genetic Variants Affect Gene Expression in Cervical Cancer [Pubmed:24945327] |
scandinavian journal of immunology | Increased Serum Levels of the IL-33 Neutralizing sST2 in Limited Cutaneous Systemic Sclerosis [PubMed: 26095613] |
Curr Eye Res | Correlation of Aqueous Humor Lysyl Oxidase Activity with TGF-ß Levels and LOXL1 Genotype in Pseudoexfoliation [Pubmed:27116380] |
journal of bone and mineral metabolism | LOX Fails to Substitute for RANKL in Osteoclastogenesis. [pubmed:27606829] |
38 | Blocking Surgically Induced Lysyl Oxidase Activity Reduces the Risk of Lung Metastases. [pubmed:28445728] |
Molecular Medicine Reports | Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritis [pubmed:28901438] |
Revista Española de Cardiología (English Edition) | A Global Assessment of Circulating Prolysyl Oxidase in Nonischemic Patients With Garden-variety Heart Failure With Preserved Ejection Fraction [Pubmed:29807761] |
Molecular Medicine Reports | Lysyl oxidase inhibition via β-aminoproprionitrile hampers human umbilical vein endothelial cell angiogenesis and migration in vitro [Pubmed:29393489] |
Thyroid | Lysyl Oxidase is a key player in BRAF/MAPK pathway-driven thyroid cancer aggressiveness [Pubmed: 30398411] |
Am J Physiol Lung Cell Mol Physiol | Lysyl Oxidase Directly Contributes to Extracellular Matrix Production and Fibrosis in Systemic Sclerosis. [Pubmed: 33026236] |
J Am Heart Assoc | Collagen Cross©\Linking Is Associated With Cardiac Remodeling in Hypertrophic Obstructive Cardiomyopathy [33356379] |
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