线粒体开放阅读框12S rRNA-C(MOTS-c)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c)
- 编号KSX132Hu11
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围4.94-400ng/mL
- 可适灵敏度
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 8316 ¥ 13860 ¥ 24948 ¥ 48510 ¥ 83160
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对线粒体开放阅读框12S rRNA-C(MOTS-c)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
SPX132Hu01 | 线粒体开放阅读框12S rRNA-C(MOTS-c)合成多肽 | Immunogen; Blocking Peptide. |
CPX132Hu21 | 线粒体开放阅读框12S rRNA-C(MOTS-c)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPX132Hu11 | 线粒体开放阅读框12S rRNA-C(MOTS-c)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAX132Hu01 | 线粒体开放阅读框12S rRNA-C(MOTS-c)多克隆抗体 | IHC; ICC; IP |
CEX132Hu | 线粒体开放阅读框12S rRNA-C(MOTS-c)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEX132Hu | 线粒体开放阅读框12S rRNA-C(MOTS-c)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMX132Hu | 线粒体开放阅读框12S rRNA-C(MOTS-c)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSX132Hu11 | 线粒体开放阅读框12S rRNA-C(MOTS-c)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Pediatric Diabetes | Circulating MOTS‐c levels are decreased in obese male children and adolescents and associated with insulin resistance [Pubmed:29691953] |
Rapid Commun Mass Spectrom | Development of a mass spectrometry‐based detection method for the mitochondrion‐derived peptide MOTS‐c in plasma samples for doping control purposes [Pubmed: 30394592] |
DIABETOLOGIA | Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via … [Pubmed: 32880686] |
Induction of hyperandrogenism and insulin resistance differentially modulates ferroptosis in uterine and placental tissues of pregnant rats [] | |
FASEB JOURNAL | Neural cell©\derived plasma exosome protein abnormalities implicate mitochondrial impairment in first episodes of psychosis [33454965] |
Biochim Biophys Acta Mol Basis Dis | MOTS-c interacts synergistically with exercise intervention to regulate PGC-1¦Á expression, attenuate insulin resistance and enhance glucose metabolism in mice via?¡ [33722744] |
researchsquare | Evolution of Mitochondrial-Derived Peptides, Humanin and MOTSc and Changes in Insulin Sensitivity During Early Gestation [] |
Mol Psychiatry | Abnormal levels of mitochondrial proteins in plasma neuronal extracellular vesicles in major depressive disorder [34471251] |
Pharmacol Res | The Mitochondrial-Derived Peptide MOTS-c Relieves Hyperglycemia and Insulin Resistance in Gestational Diabetes Mellitus [34798268] |
Ann Neurol | SARS‐CoV‐2 and mitochondrial proteins in neural‐derived exosomes of COVID‐19 [Pubmed:35285072] |
J Clin Med | Evolution of Mitochondrially Derived Peptides Humanin and MOTSc, and Changes in Insulin Sensitivity during Early Gestation in Women with and without Gestational … [Pubmed:35683389] |
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