硝基酪氨酸(NT)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Nitrotyrosine (NT)
3-Nitro-L-Tyrosine; 3-Nitrotyrosine
- 编号KSB863Ge11
- 物种Pan-species (General,通用) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围9.88-800ng/mL
- 可适灵敏度3.95ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 8726 ¥ 14544 ¥ 26179 ¥ 50904 ¥ 87264
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对硝基酪氨酸(NT)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Pan-species (General,通用) | 应用(仅供研究使用,不用于临床诊断!) |
CPB863Ge11 | 硝基酪氨酸(NT)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPB863Ge21 | 硝基酪氨酸(NT)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAB863Ge01 | 硝基酪氨酸(NT)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
CEB863Ge | 硝基酪氨酸(NT)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEB863Ge | 硝基酪氨酸(NT)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB863Ge | 硝基酪氨酸(NT)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB863Ge11 | 硝基酪氨酸(NT)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Molecular Medicine Reports | 3-Nitrotyrosine, a biomarker for cardiomyocyte apoptosis induced by diabetic cardiomyopathy in a rat model [Pubmed: 23970331] |
Free Radical Biology and Medicine | Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835] |
J Histochem Cytochem | Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats [PubMed: 26385569] |
canadian journal of physiology and pharmacology | Protective effects of Telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuro-inflammation and amyloidogenesis: possible role of brain derived neurotrophic factor [10.1139:cjpp-2017-0042] |
Orphanet journal of rare diseases | The cardiovascular phenotype of adult patients with phenylketonuria [Pubmed: 31492166] |
ACTA PHARMACOLOGICA SINICA | The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice [33303990] |
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