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头蛋白(NOG)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Noggin (NOG)

SYM1; SYNS1; Synostoses(Multiple)Syndrome 1; Symphalangism 1(Proximal)

  • 头蛋白(NOG)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 头蛋白(NOG)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对头蛋白(NOG)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
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LAC130Hu76 头蛋白(NOG)单克隆抗体(生物素标记) WB; IHC; ICC.
SEC130Hu 头蛋白(NOG)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC130Hu 头蛋白(NOG)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
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KSC130Hu01 头蛋白(NOG)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Arthritis Rheumatol Imbalance between BMP2 and Noggin induces abnormal osteogenic differentiation of mesenchymal stem cells in ankylosing spondylitis [PubMed: 26413886]
Arthritis Rheumatol.  Imbalance Between Bone Morphogenetic Protein 2 and Noggin Induces Abnormal Osteogenic Differentiation of Mesenchymal Stem Cells in Ankylosing Spondylitis [Pubmed:26413886]
Clin Exp Pharmacol Physiol. Oxygen-glucose deprivation preconditioning protects neurons against oxygen-glucose deprivation/reperfusion induced injury via bone morphogenetic protein-7 mediated ERK, p38 and Smad signalling pathways. [Pubmed:26385023]
European cells & materials The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells. [pubmed:28266688]
Arthritis & Rheumatology mbalance Between Bone Morphogenetic Protein 2 and Noggin Induces Abnormal OsteogenicDifferentiation of Mesenchymal Stem Cells in Ankylosing Spondylitis. [pubmed:26413886]
Biotechnology and Applied Biochemistry The BMP antagonist Noggin is produced by osteoblasts in response to the presence of prostate cancer cells. [pubmed:28981962]
Heliyon Efficient in vitro delivery of Noggin siRNA enhances osteoblastogenesis [pubmed:29167826]
Journal of Cellular Biochemistry Low‐intensity pulsed ultrasound promotes bone morphogenic protein 9‐induced osteogenesis and suppresses inhibitory effects of inflammatory cytokines on cellular … [Pubmed: 31006911]
Aging Male Effect of tadalafil on penile nitric oxide synthase and corporal smooth muscle in rats under dutasteride treatment [Pubmed: 32160825]
J Reconstr Microsurg Effect of Simvastatin Use in Free Tissue Transfer: An Experimental Study in a Rat Epigastric Free Flap Model [Pubmed: 31995818]
Biochem Biophys Rep Slow diffusion on the monolayer culture enhances auto/paracrine effects of Noggin in differentiation of human iPS cells induced by BMP [Pubmed:35005256]
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