Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Procollagen I N-Terminal Propeptide (PINP)
P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide
- 编号KSA957Hu11
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围2.47-200ng/mL
- 可适灵敏度0.91ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对Ⅰ型前胶原氨基端原肽(PINP)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPA957Hu02 | Ⅰ型前胶原氨基端原肽(PINP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA957Hu61 | Ⅰ型前胶原氨基端原肽(PINP)活性蛋白 | Cell culture; Activity Assays. |
RPA957Hu01 | Ⅰ型前胶原氨基端原肽(PINP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA957Hu61 | Ⅰ型前胶原氨基端原肽(PINP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
SPA957Hu02 | Ⅰ型前胶原氨基端原肽(PINP)合成多肽 | Immunogen; Blocking Peptide. |
CPA957Hu12 | Ⅰ型前胶原氨基端原肽(PINP)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA957Hu32 | Ⅰ型前胶原氨基端原肽(PINP)钥孔血蓝蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA957Hu31 | Ⅰ型前胶原氨基端原肽(PINP)钥孔血蓝蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA957Hu22 | Ⅰ型前胶原氨基端原肽(PINP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA957Hu23 | Ⅰ型前胶原氨基端原肽(PINP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA957Hu21 | Ⅰ型前胶原氨基端原肽(PINP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
SPA957Hu01 | Ⅰ型前胶原氨基端原肽(PINP)合成多肽 | Immunogen; Blocking Peptide. |
CPA957Hu11 | Ⅰ型前胶原氨基端原肽(PINP)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAA957Hu08 | Ⅰ型前胶原氨基端原肽(PINP)多克隆抗体 | WB; IHC; ICC; IP. |
PAA957Hu09 | Ⅰ型前胶原氨基端原肽(PINP)多克隆抗体 | WB; IHC; ICC; IP. |
PAA957Hu01 | Ⅰ型前胶原氨基端原肽(PINP)多克隆抗体 | WB; IHC; ICC; IP. |
PAA957Hu02 | Ⅰ型前胶原氨基端原肽(PINP)多克隆抗体 | WB; IHC; ICC; IP. |
LAA957Hu81 | Ⅰ型前胶原氨基端原肽(PINP)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAA957Hu71 | Ⅰ型前胶原氨基端原肽(PINP)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAA957Hu22 | Ⅰ型前胶原氨基端原肽(PINP)单克隆抗体 | WB |
RAA957Hu23 | Ⅰ型前胶原氨基端原肽(PINP)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
RAA957Hu22 | Ⅰ型前胶原氨基端原肽(PINP)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
MAA957Hu24 | Ⅰ型前胶原氨基端原肽(PINP)单克隆抗体 | WB; IHC; ICC; IP. |
MAA957Hu21 | Ⅰ型前胶原氨基端原肽(PINP)单克隆抗体 | WB,IHC |
MAA957Hu28 | Ⅰ型前胶原氨基端原肽(PINP)单克隆抗体 | WB; IHC |
MAA957Hu23 | Ⅰ型前胶原氨基端原肽(PINP)单克隆抗体 | WB; IHC; ICC; IP. |
MAA957Hu25 | Ⅰ型前胶原氨基端原肽(PINP)单克隆抗体 | WB; IHC; ICC; IP. |
MAA957Hu27 | Ⅰ型前胶原氨基端原肽(PINP)单克隆抗体 | WB; IHC; ICC; IP. |
MAA957Hu29 | Ⅰ型前胶原氨基端原肽(PINP)单克隆抗体 | WB; IHC; ICC; IP. |
SEA957Hu | Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
IEA957Hu | Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒(酶联免疫吸附试验法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA957Hu | Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA957Hu | Ⅰ型前胶原氨基端原肽(PINP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSA957Hu01 | Ⅰ型前胶原氨基端原肽(PINP)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA957Hu11 | Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
KSA957Hu01 | Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
Trials | Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial. [Trials: 1745-6215] |
Clinical Biochemistry | Measurement of aminoterminal propeptide of type I procollagen (PINP) in serum [ScienceDirect: S0009912012001543] |
Therapeutic Apheresis and Dialysis | Low molecular weight iron dextran increases fibroblast growth factor-23 concentration, together with parathyroid hormone decrease in hemodialyzed patients [PubMed: 22458393] |
International Journal of Clinical & Experimental Pathology | IL4 and IL-17A provide a Th2/Th17-polarized inflammatory milieu in favor of TGF-β1 to induce bronchial epithelial-mesenchymal transition (EMT) [PubMed: PMC3726963] |
Osteoporosis International | PINP as a biological response marker during teriparatide treatment for osteoporosis [Pubmed: 24599274] |
Clinical Cancer Research | Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivo [Aacrjournals: Source] |
Endocrinology | Oxytocin Reverses Ovariectomy-Induced Osteopenia and Body Fat Gain [Endocrine: Source] |
general endocrinology | Oxytocin reverse ovariectomy-induced Osteoper and body fat gain [Pubmed:24506069] |
J Neurol Sci. | The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism. [Pubmed:24629477] |
Bone. | Neurofibromin inactivation impairs osteocyte development in Nf1Prx1 and Nf1Col1 mouse models [Pubmed:24947449] |
Journal of Materials Science: Materials in Medicine | Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesis [Pubmed:24980874] |
Springerplus. | Effects of de-escalated bisphosphonate therapy on bone turnover biomarkers in breast cancer patients with bone metastases [Pubmed:25332877] |
Pharmacology | Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy [PubMed: 25871861] |
frontiers in endocrinology | Oxytocin reverses osteoporosis in a sex-dependent manner [PubMed: 26042090] |
MERIT RESEARCH JOURNALS | Changes in Bone Metabolism Months after Laparoscopic Morbidly Obese Pre-menopausal Women [Mms: Content] |
Heart Rhythm. | Prognostic value of collagen turnover biomarkers in cardiac resynchronization therapy: A subanalysis of the TRUST CRT randomized trial population [Pubmed:26776557] |
BMJ Open Sport Exerc Med | Procollagen markers in microdialysate can predict patient outcome after Achilles tendon rupture [content:2] |
Bibliothekssystem Universität Hamburg | Bilaterale Kommunikation zwischen Osteoblasten und Osteoklasten [volltexte:2016] |
Inflammation Research | Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy [pubmed:27516211] |
Endocrine | Bone turnover response is linked to both acute and established metabolic changes in ultra-marathon runners [pubmed:27422791] |
Heart & Vessels | Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [pubmed:28004175] |
journal of science and medicine in sport | The effect of calcium and vitamin D supplementation on bone health of male Jockeys. [pubmed:27568072] |
Combined aerobic and resistance training improves bone health of female cancer survivors [S2352187216300389] | |
Cellular Physiology and Biochemistry | Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy [pubmed:27889753] |
Epilepsy Research | The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats [pubmed:27838501] |
Sci Rep. | Sprint Interval Training Induces A Sexual Dimorphism but does not Improve Peak Bone Mass in Young and Healthy Mice. [pubmed:28303909] |
Metabolism. | Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity. [pubmed:28521873] |
Heart and vessels | Erratum to: Effects on bone metabolism markers and arterial stiffness by switching to rivaroxaban from warfarin in patients with atrial fibrillation [pubmed:28314975] |
SCIENCE & TECHNOLOGY | A Study Protocol: Spinal Morphology, Physical Performance, Quality of Life and Biochemical Markers in Adults at Risk of Osteoporotic Fractures [59f7c85aaca272607e2d8d7a] |
Knee Surg Sports Traumatol Arthrosc | Achilles tendon rupture healing is enhanced by intermittent pneumatic compression upregulating collagen type I synthesis [pubmed:28668970] |
Method for the treatment of cardiovascular fibrosis [patents:US9594083] | |
Clinical Cases in Mineral and Bone Metabolism | Periostin and sclerostin levels in juvenile Paget's disease [pubmed:29263750] |
Journal of Clinical Research in Pediatric Endocrinology | Congenital Hypothyroidism and Bone Remodeling Cycle [pubmed:27840329] |
Biomarkers Medicial | Relationship of biomarkers of extracellular matrix with myocardial function in Type 2 diabetes mellitus [pubmed:28685602] |
Heart and Vessels | Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [10.1007/s00380-016-0930-y] |
BioMed Research International | Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research [pubmed:29119115] |
Cytokine | 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis. [pubmed:28460256] |
Advanced Healthcare Materials | Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. [pubmed:29120858] |
Bone. | The key role of proinflammatory cytokines, matrix proteins, RANKL/OPG and Wnt/β-catenin in bone healing of hip arthroplasty patients [pubmed:29129760] |
Cell Death And Differentiation | TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42 [Pubmed:29358671] |
Nutrients | Specific Collagen Peptides Improve Bone Mineral Density and Bone Markers in Postmenopausal Women—A Randomized Controlled Study [Pubmed:29337906] |
Osteoporosis International | Two-year cortical and trabecular bone loss in CKD-5D: biochemical and clinical predictors [Pubmed:28993865] |
Journal of Nutrition | Cholecalciferol Supplementation Promotes Bone Turnover in Chinese Adults with Vitamin D Deficiency [Pubmed:29897564] |
European Journal of Pharmacology | The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism [Pubmed:29428468] |
Journal of Clinical Densitometry | Geometric and mechanical bone response to a multidisciplinary weight loss intervention in adolescents with obesity: The ADIBOX study [Pubmed:30076009] |
Hernia | Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia [Pubmed:29484522] |
Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457] |
Nature | Coupling of bone resorption and formation by RANKL reverse signalling [Pubmed: 30185903] |
European Journal of Medical Research | Biomechanical and histological analyses of the fracture healing process after direct or prolonged reduction [Pubmed: 30180907] |
Sensors | Membrane-Based Microwave-Mediated Electrochemical Immunoassay for the In Vitro, Highly Sensitive Detection of Osteoporosis-Related Biomarkers [Pubmed: 30181433] |
Trials | Structured diet and exercise guidance in pregnancy to improve health in women and their offspring: study protocol for the Be Healthy in Pregnancy (BHIP) … [Pubmed: 30567604] |
International Journal of Cancer | Effect of induction therapy with lenalidomide, doxorubicin, and dexamethasone on bone remodeling and angiogenesis in newly diagnosed multiple myeloma [Pubmed: 30650184] |
Bone | Rapamycin improves bone mass in high-turnover osteoporosis with iron accumulation through positive effects on osteogenesis and angiogenesis [Pubmed: 30610968] |
Revista de Chimie -Bucharest- | Quantification of N-Terminal Procollagen I Propeptide in Patients With Occlusal Trauma Induced by Prosthetic Factors [] |
PLOS ONE | Effect of antibiotic infused calcium sulfate/hydroxyapatite (CAS/HA) insets on implant-associated osteitis in a femur fracture model in mice [Pubmed: 30870491] |
Scientific Reports | Low fibrosis biomarker levels predict cardiac resynchronization therapy response [Pubmed: 30988339] |
Current Developments in Nutrition | Changes in Calciotropic Hormones and Bone Markers During Pregnancy in Response to a Nutrition+ Exercise Intervention in the Be Healthy in Pregnancy RCT (P11 … [Pubmed: 31224444] |
Stem Cell Research & Therapy | Low concentration flufenamic acid enhances osteogenic differentiation of mesenchymal stem cells and suppresses bone loss by inhibition of the NF-κB … [Pubmed: 31324207] |
Bone | The long noncoding RNA Crnde regulates osteoblast proliferation through the Wnt/β-catenin signaling pathway in mice [Pubmed: 31622775] |
International Journal of Basic and Clinical Endocrinology | Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia [Pubmed: 30143940] |
Bone | Mice lacking plastin-3 display a specific defect of cortical bone acquisition [Pubmed: 31678489] |
International journal of molecular sciences | Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic Patients [Pubmed: 31717649] |
Journal of Bone and Mineral Research | BMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodeling [Pubmed: 31914211] |
ESC Heart Failure | Outcome in German and South African peripartum cardiomyopathy cohorts associates with medical therapy and fibrosis markers [Pubmed: 32064780] |
International Journal of Medical Sciences | Hypoalbuminemia differently affects the serum bone turnover markers in hemodialysis patients [Pubmed: 31839746] |
JOURNAL OF BONE AND MINERAL RESEARCH | Piezo1 Inactivation in Chondrocytes Impairs Trabecular Bone Formation [Pubmed: 33180356] |
JOURNAL OF THROMBOSIS AND THROMBOLYSIS | Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertension [Pubmed: 33175289] |
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | Development of a novel polysaccharide-based iron oxide nanoparticle to prevent iron accumulation-related osteoporosis by scavenging reactive oxygen … [Pubmed: 33049237] |
journal of bone and mineral research | Mice carrying a ubiquitous R235W mutation of Wnt1 display a bone‐specific phenotype [Pubmed: 32369212] |
Endokrynologia Polska | FGF23 and primary hyperparathyroidism: is there a link? [Pubmed: 32598019] |
Journal of Orthopaedic Translation | Qu Feng Zhi Tong capsule increases mechanical properties of cortical bone in ovariectomized rats [] |
Research square | 4-Hexylresorcinol Inhibits Osteoclastogenesis by Suppressing NF‐κB Signaling Pathway and Reverses Bone Loss in Ovariectomized Mice [] |
淫羊藿水提物及提取药渣的抗骨质疏松药效比较研究 [] | |
Pamukkale Tıp Dergisi | Postmenapozal Tip-2 diyabetes mellitus' lu hastalarda osteoporoza bağlı kırık riskinin ve kemik Turnoveri'nin değerlendirilmesi [] |
International Journal of Pharmaceutical and Clinical Research | Bone Turnover Markers in Osteogenesis Imperfecta and Effect of Bisphosphonate Treatment: First Egyptian Study [] |
Science Advances | A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair [33310848] |
Rheumatology | Proinflammatory and bone protective role of calcitonin gene-related peptide alpha in collagen antibody-induced arthritis [33221885] |
Nutrients | Low Energy Availability with and without a High-Protein Diet Suppresses Bone Formation and Increases Bone Resorption in Men: A Randomized Controlled Pilot ¡ [33671093] |
J Bone Miner Res | Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/¦Â©\catenin pathway [33950533] |
Phytomedicine | Randomized, double-blind, placebo-controlled trial to examine the safety, pharmacokinetics and effects of Epimedium prenylflavonoids, on bone specific alkaline … [34352588] |
Nutrients | The Effect of Daily Methylsulfonylmethane (MSM) Consumption on High-Density Lipoprotein Cholesterol in Healthy Overweight and Obese Adults: A Randomized … [34684621] |
PLoS One | Osteoblast-specific inactivation of p53 results in locally increased bone formation [34793446] |
Bone Res | The WNT1G177C mutation specifically affects skeletal integrity in a mouse model of osteogenesis imperfecta type XV [34759273] |
Nutrients | The Effect of Caloric Restriction with and without n-3 PUFA Supplementation on Bone Turnover Markers in Blood of Subjects with Abdominal Obesity: A Randomized … [34578973] |
Chronobiol Int | Night shift work and osteoporosis-bone turnover markers among female blue-collar workers in Poland [Pubmed:35139709] |
Cancers (Basel) | Spine Metastases in Immunocompromised Mice after Intracardiac Injection of MDA-MB-231-SCP2 Breast Cancer Cells [Pubmed:35158823] |
Cell Death Discov | BMP9 reduces age-related bone loss in mice by inhibiting osteoblast senescence through Smad1-Stat1-P21 axis [Pubmed:35523787] |
Experimental and Molecular Medicine | Foxf2 represses bone formation via Wnt2b/β-catenin signaling [Pubmed:35668101] |
Bioactive Materials | Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles [Pubmed:35386445] |
Journal of Orthopaedic Translation | Puerarin specifically disrupts osteoclast activation via blocking integrin-β3 Pyk2/Src/Cbl signaling pathway [Pubmed:35228997] |
Bioactive Materials | Construction of a magnesium hydroxide/graphene oxide/hydroxyapatite composite coating on Mg–Ca–Zn–Ag alloy to inhibit bacterial infection and promote … [Pubmed:35585377] |
Journal of Orthopaedic Translation | Exercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the Gpr35 … [] |
留言咨询