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抗增殖细胞核抗原(PCNA)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Proliferating Cell Nuclear Antigen (PCNA)

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  • 抗增殖细胞核抗原(PCNA)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 抗增殖细胞核抗原(PCNA)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对抗增殖细胞核抗原(PCNA)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPA591Hu01 抗增殖细胞核抗原(PCNA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CAA591Hu01 抗抗增殖细胞核抗原(PCNA)多克隆抗体 Loading Control of WB.
PAA591Hu01 抗增殖细胞核抗原(PCNA)多克隆抗体 WB; IHC; ICC; IP.
MAA591Hu21 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
MAA591Hu22 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
MAA591Hu23 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
MAA591Hu25 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
MAA591Hu24 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
CAA591Hu22 抗抗增殖细胞核抗原(PCNA)单克隆抗体 Loading Control of WB.
KSA591Hu01 抗增殖细胞核抗原(PCNA)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Food and Chemical Toxicology Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic rats [Pubmed:30026088]
Life Sciences Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats [Pubmed: 30205096]
Archives of Physiology and Biochemistry Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin [Pubmed: 30513216]
European journal of medicinal chemistry Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity [Pubmed: 31216504]
Antioxidants (Basel). The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets [Pubmed: 31426309]
Antioxidants Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian … [Pubmed: 31600920]
TOXICOLOGY Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis [Pubmed: 32565124]
BIOMEDICINE & PHARMACOTHERAPY Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats [Pubmed: 33152939]
Sci Rep Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats [33750916]
J Cell Sci Local DNA synthesis is critical for DNA repair during oocyte maturation [34415018]
Stem Cell Rev Rep Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic … [34859376]
Naunyn-Schmiedeberg's Archives Inflammatory Modulation of miR-155 Inhibits Doxorubicin‑Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus … [Pubmed:35715546]
Antioxidants Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model … [Pubmed:35204140]
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … []
Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation … []
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