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蛋白激酶Cβ1(PKCb1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Protein Kinase C Beta 1 (PKCb1)

PKC-B1; PRKCB; PRKCB1

  • 蛋白激酶Cβ1(PKCb1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 蛋白激酶Cβ1(PKCb1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对蛋白激酶Cβ1(PKCb1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Oryctolagus cuniculus (Rabbit,兔) 应用(仅供研究使用,不用于临床诊断!)
RPB045Rb01 蛋白激酶Cβ1(PKCb1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB045Rb51 蛋白激酶Cβ1(PKCb1)多克隆抗体 WB; IHC; ICC; IP.
PAB045Rb01 蛋白激酶Cβ1(PKCb1)多克隆抗体 WB; IHC; ICC; IP.
LAB045Rb71 蛋白激酶Cβ1(PKCb1)多克隆抗体(生物素标记) WB; IHC; ICC.
KSB045Rb01 蛋白激酶Cβ1(PKCb1)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Vascular Pharmacology Hesperetin ameliorates hyperglycemia induced retinal vasculopathy via anti-angiogenic effects in experimental diabetic rats [ScienceDirect: S1537189112000407]
Food and Chemical Toxicology Prevention and management of diabetic retinopathy in STZ diabetic rats by Tinospora cordifolia and its molecular mechanisms [PubMed: S0278691512004103]
Journal of ocular pharmacology and therapeutics Retinoprotective Effects of Moringa oleifera via Antioxidant, Anti-Inflammatory, and Anti-Angiogenic Mechanisms in Streptozotocin-Induced Diabetic Rats [Pubmed: 23215831]
Mol Cell Biochem. Effects of Trigonella foenum-graecum (L.) on retinal oxidative stress, and proinflammatory and angiogenic molecular biomarkers in streptozotocin-induced diabetic rats [Pubmed:24242137]
J Clin Diagn Res. Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic Rats Supplemented with Momordica charantia [pmc:PMC4866090]
Journal of Clinical&Diagnostic Research Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic RatsSupplemented with Momordica charantia. [pubmed:27190792]
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