蛋白激酶Cβ1(PKCb1)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Protein Kinase C Beta 1 (PKCb1)
PKC-B1; PRKCB; PRKCB1
- 编号KSB045Rb01
- 物种Oryctolagus cuniculus (Rabbit,兔) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.061ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7445 ¥ 12408 ¥ 22334 ¥ 43428 ¥ 74448
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特异性
试剂盒中的抗体对蛋白激酶Cβ1(PKCb1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Oryctolagus cuniculus (Rabbit,兔) | 应用(仅供研究使用,不用于临床诊断!) |
RPB045Rb01 | 蛋白激酶Cβ1(PKCb1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB045Rb51 | 蛋白激酶Cβ1(PKCb1)多克隆抗体 | WB; IHC; ICC; IP. |
PAB045Rb01 | 蛋白激酶Cβ1(PKCb1)多克隆抗体 | WB; IHC; ICC; IP. |
LAB045Rb71 | 蛋白激酶Cβ1(PKCb1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
KSB045Rb01 | 蛋白激酶Cβ1(PKCb1)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
Vascular Pharmacology | Hesperetin ameliorates hyperglycemia induced retinal vasculopathy via anti-angiogenic effects in experimental diabetic rats [ScienceDirect: S1537189112000407] |
Food and Chemical Toxicology | Prevention and management of diabetic retinopathy in STZ diabetic rats by Tinospora cordifolia and its molecular mechanisms [PubMed: S0278691512004103] |
Journal of ocular pharmacology and therapeutics | Retinoprotective Effects of Moringa oleifera via Antioxidant, Anti-Inflammatory, and Anti-Angiogenic Mechanisms in Streptozotocin-Induced Diabetic Rats [Pubmed: 23215831] |
Mol Cell Biochem. | Effects of Trigonella foenum-graecum (L.) on retinal oxidative stress, and proinflammatory and angiogenic molecular biomarkers in streptozotocin-induced diabetic rats [Pubmed:24242137] |
J Clin Diagn Res. | Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic Rats Supplemented with Momordica charantia [pmc:PMC4866090] |
Journal of Clinical&Diagnostic Research | Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic RatsSupplemented with Momordica charantia. [pubmed:27190792] |
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