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调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)

CCL5; SCYA5; SISd; EoCP; Chemokine C-C-Motif Ligand 5; Eosinophil Chemotactic Cytokine; T-cell Specific Protein p288; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5

  • 调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对调节激活正常T-细胞表达分泌因子(RANTES)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Bos taurus; Bovine (Cattle，牛) 应用(仅供研究使用，不用于临床诊断！)
RPA116Bo01 调节激活正常T-细胞表达分泌因子(RANTES)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA116Bo01 调节激活正常T-细胞表达分泌因子(RANTES)多克隆抗体 WB; IHC; ICC; IP.
LAA116Bo81 调节激活正常T-细胞表达分泌因子(RANTES)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA116Bo71 调节激活正常T-细胞表达分泌因子(RANTES)多克隆抗体(生物素标记) WB; IHC; ICC.
KSA116Bo01 调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
J Thorac Dis.? Anti-endothelial cell antibodies in connective tissue diseases associated with pulmonary arterial hypertension [Pubmed:Pmc4015009]
Pharmacol Rep.? Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression [Pubmed:25150002]
Pharmacological Reports The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders [Pubmed:25560580]
Pharmacological Reports Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder [PubMed: 26481549]
Veterinary Parasitology Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis [PubMed: 26412140]
Pharmacological Reports A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity [Pubmed:27208878]
PLoS One.  Diacerein inhibits the pro-atherogenic & pro-inflammatory effects of IL-1 on human keratinocytes & endothelial cells. [pubmed:28323859]
Experimental Parasitology Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis [pubmed:28821422]
National Natural Scientific Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure [pubmed:29121396]
Journal of Immunology Research Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis Rat [Pubmed:29682587]
Rheumatology international Serum levels of selected cytokines [interleukin (IL)-17A, IL-18, IL-23] and chemokines (RANTES, IP10) in the acute phase of immunoglobulin A vasculitis in … [Pubmed: 31468124]
Cell Death & Disease CCL5 derived from tumor-associated macrophages promotes prostate cancer stem cells and metastasis via activating β-catenin/STAT3 signaling [Pubmed: 32300100]
Medicina Assessment of the RANTES Level Correlation and Selected Inflammatory and Pro-Angiogenic Molecules Evaluation of Their Influence on CRC Clinical Features: A … [Pubmed:35208526]
Journal of Immunology Research Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes [Pubmed:35340587]
Reseach square Assessment of selected interleukins (IL-6, IL-17A, IL-18, IL-23) and chemokines (RANTES, IP-10) in children with acute and chronic urticaria []
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