山梨醇脱氢酶(SDH)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Sorbitol Dehydrogenase (SDH)
SORD; L-iditol 2-dehydrogenase
- 编号KSB495Ra01
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围1.56-100ng/mL
- 可适灵敏度0.66ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对山梨醇脱氢酶(SDH)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPB495Ra01 | 山梨醇脱氢酶(SDH)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB495Ra01 | 山梨醇脱氢酶(SDH)多克隆抗体 | WB; IHC; ICC; IP. |
MAB495Ra21 | 山梨醇脱氢酶(SDH)单克隆抗体 | WB; IHC; ICC; IP. |
SEB495Ra | 山梨醇脱氢酶(SDH)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB495Ra | 山梨醇脱氢酶(SDH)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB495Ra01 | 山梨醇脱氢酶(SDH)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Kafkas Univ Vet Fak Derg | The Importance of Concentrations of Sorbitol Dehydrogenase and Glutamate Dehydrogenase and B-Mode Ultrasonographic Examination in The Diagnosis of Hepatic Lipidosis in Dairy Cows [Kafkas: Source] |
Journal of Food Science and Technology | Grape seed extract and Zinc containing nutritional food supplement delays onset and progression of Streptozocin-induced diabetic cataract in Wistar rats [Springer: Source] |
Clinical and Experimental Optometry | Aldo-keto reductase and sorbitol dehydrogenase enzymes in Egyptian diabetic patients with and without proliferative diabetic retinopathy. [Pubmed: 23452182] |
Biomedicine & Pharmacotherapy | Zinc/alogliptin combination attenuates testicular toxicity induced by doxorubicin in rats: Role of oxidative stress, apoptosis and TGF-β1/NF-κB signaling [pubmed:29091894] |
Molecular Medicine Reports | Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion [Pubmed:29393473] |
Effect of the combination between empagliflozin and calcipotriol on cadmium-induced testicular toxicity in rats [] |
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