超氧化物歧化酶1(SOD1)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Superoxide Dismutase 1 (SOD1)
ALS; ALS1; IPOA; Superoxide Dismutase 1, Soluble; Amyotrophic Lateral Sclerosis 1,Adult; Superoxide dismutase [Cu-Zn]
- 编号KSB960Mu01
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.067ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对超氧化物歧化酶1(SOD1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPB960Mu02 | 超氧化物歧化酶1(SOD1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB960Mu01 | 超氧化物歧化酶1(SOD1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB960Mu01 | 超氧化物歧化酶1(SOD1)活性蛋白 | Cell culture; Activity Assays. |
PAB960Mu01 | 超氧化物歧化酶1(SOD1)多克隆抗体 | WB; IHC; ICC; IP. |
PAB960Mu02 | 超氧化物歧化酶1(SOD1)多克隆抗体 | WB; IHC; ICC; IP. |
LAB960Mu81 | 超氧化物歧化酶1(SOD1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAB960Mu71 | 超氧化物歧化酶1(SOD1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
SEB960Mu | 超氧化物歧化酶1(SOD1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB960Mu | 超氧化物歧化酶1(SOD1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB960Mu01 | 超氧化物歧化酶1(SOD1)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
AGE | Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closure [Pubmed:24443098] |
Cardiovascular Diabetology | Diabetic cardiomyopathy is associated with defective myocellular copper regulation and both defects are rectified by divalent copper chelation [Pubmed:24927960] |
Arch Oral Biol | Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes [PubMed: 26143097] |
Oxidative Medicine and Cellular Longevity | BQ123 Stimulates Skeletal Muscle Antioxidant Defense via Nrf2 Activation in LPS-Treated Rats [Journals: Omcl] |
О Р И Г И Н А Л Ь Н Ы Е С Т А Т Ь И | КЛИНИЧЕСКОЕ ЗНАЧЕНИЕ ФАКТОРОВ КЛЕТОЧНОЙ РЕГУЛЯЦИИ И ФЕРМЕНТОВ АНТИОКСИДАНТНОЙ СИСТЕМЫ У ПАЦИЕНТОК РЕПРОДУКТИВНОГО ВОЗРАСТА С НАРУЖНЫМ ГЕНИТАЛЬНЫМ ЭНДОМЕТРИ [docs:225188] |
Evidence-Based Complementary and Alternative Medicine | Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariae [pmc:PMC4794592] |
Free Radic Biol Med. | DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts. [Pubmed:26806551] |
Adv Med Sci. | Orthodontic treatment modifies the oxidant-antioxidant balance in saliva of clinically healthy subjects. [pubmed:28242484] |
Iranian Journal of Kidney Diseases | Clinical Characterization of Serum Docosahexaenoic Acid and Its Relationship With Inflammation Factors in Patients With Diabetic Nephropathy [Pubmed:29507271] |
Journal of Integrative Medicine | Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats [Doi: 10.1016/j.joim.2018.12.002] |
Metallomics | Restoration of myocellular copper-trafficking proteins and mitochondrial copper enzymes repairs cardiac function in rats with diabetes-evoked heart failure [Pubmed: 31821401] |
JOURNAL OF PHARMACY AND PHARMACOLOGY | Grape seed extract improved the fertility-enhancing effect of atorvastatin in high-fat diet-induced testicular injury in rats: involvement of antioxidant and anti-apoptotic?¡ [33793875] |
ACS Omega | SOD1 is a Possible Predictor of COVID-19 Progression as Revealed by Plasma Proteomics [34250342] |
Korean J Physiol Pharmacol | Improved motility in the gastrointestinal tract of a postoperative ileus rat model with ilaprazole [34697261] |
Pharmaceuticals | Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients [34832849] |
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