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线粒体超氧化物歧化酶(SOD2)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Superoxide Dismutase 2, Mitochondrial (SOD2)

IPO-B; MNSOD; Mn-SOD

  • 线粒体超氧化物歧化酶(SOD2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 线粒体超氧化物歧化酶(SOD2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对线粒体超氧化物歧化酶(SOD2)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
APB083Ra01 线粒体超氧化物歧化酶(SOD2)活性蛋白 Cell culture; Activity Assays.
RPB083Ra01 线粒体超氧化物歧化酶(SOD2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB083Ra01 线粒体超氧化物歧化酶(SOD2)多克隆抗体 WB,IHC,ICC/IF
LAB083Ra71 线粒体超氧化物歧化酶(SOD2)多克隆抗体(生物素标记) WB; IHC; ICC.
LAB083Ra81 线粒体超氧化物歧化酶(SOD2)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAB083Ra21 线粒体超氧化物歧化酶(SOD2)单克隆抗体 WB; IHC; ICC; IP.
SEB083Ra 线粒体超氧化物歧化酶(SOD2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB083Ra 线粒体超氧化物歧化酶(SOD2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB083Ra01 线粒体超氧化物歧化酶(SOD2)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
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AGE Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closure [Pubmed:24443098]
Anticancer Research Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal Cancer [Pubmed:25550558]
Arch Oral Biol Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes [PubMed: 26143097]
J Oral Pathol Med Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva [PubMed: 26608886]
Free Radic Biol Med. DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts. [Pubmed:26806551]
Basic Research in Cardiology Cardiomyocyte dimethylarginine dimethylaminohydrolase1 attenuates left-ventricular remodeling after acute myocardial infarction: involvement in oxidative stress and … [Pubmed:29892894]
Journal of Pharmacological Sciences Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome [Pubmed: 30342783]
Advances in Medical Sciences Selected elements of extracellular matrix of the skin in diabetes and insulin resistance [Pubmed: 31146169]
PHARMACOLOGICAL RESEARCH A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 Signaling [Pubmed: 32135248]
Biomolecules Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development [Pubmed: 32751983]
Nutrients Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and?¡­ [34064937]
Pharmaceuticals Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients [34832849]
Biochimica et Biophysica Acta-Molecular Basis of Disease α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat diet [Pubmed:35569738]
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