表面活性物质关联蛋白B(SP-B)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Surfactant Protein B (SP-B)
SFTPB; PSPB; SFTB3; SFTP3; SPB; Surfactant Associated Protein B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein
- 编号KSB622Ra01
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Tissue homogenates, cell lysates, lung lavage fluid and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.054ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对表面活性物质关联蛋白B(SPB)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB622Ra01 | 表面活性物质关联蛋白B(SP-B)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB622Ra01 | 表面活性物质关联蛋白B(SP-B)多克隆抗体 | WB; IHC; ICC; IP. |
| MAB622Ra21 | 表面活性物质关联蛋白B(SP-B)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB622Ra | 表面活性物质关联蛋白B(SP-B)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB622Ra | 表面活性物质关联蛋白B(SP-B)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB622Ra01 | 表面活性物质关联蛋白B(SP-B)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Respiratory Physiology & Neurobiology | Kinetics of plasma SPB and RAGE during mechanical ventilation in patients undergoing major vascular surgery [PubMed: 21736957] |
| American Journal of Rhinology & Allergy | Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps [PubMed: 23406594] |
| 19 | Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: a pilot study [PubMed: 20650982] |
| Plos one | Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins [PubMed: PMC3551896] |
| Plos one | The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS [PubMed: PMC3787032] |
| Cancer Cell. | Lung Cancer Signatures in Plasma Based on Proteome Profiling of Mouse Tumor Models [PubMed: PMC3406925] |
| Respir Physiol Neurobiol | Acute high-altitude exposure reduces lung diffusion: Data from the HIGHCARE Alps project [Pubmed: 23619193] |
| PLOS ONE | Surfactant-Derived Proteins as Markers of Alveolar Membrane Damage in Heart Failure [Pubmed:25514679] |
| Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge [Opus4:Source] | |
| Int J Cardiol | Plasma immature form of surfactant protein type B correlates with prognosis in patients with chronic heart failure. A pilot single-center prospective study [PubMed: 26310985] |
| Plos One | Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE) [Pubmed:27337142] |
| PLoS One | The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions. [pubmed:27656877] |
| Frontiers in Aging Neuroscience | Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid. [pubmed:28101052] |
| Frontiers in Aging Neuroscience | Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid [PMC5209370] |
| Respiratory Physiology & Neurobiology | Diving and pulmonary physiology: surfactant binding protein, lung fluid and cardiopulmonary test changes in professional divers [pubmed:28467885] |
| Cellular Physiology and Biochemistry | Surfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid Production [pubmed:28743125] |
| American Journal of Physiology. Lung Cellular and Molecular Physiology | Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency. [pubmed:29167126] |
| Respiratory research | Effects and molecular mechanisms of intrauterine infection/inflammation on lung development [Pubmed:29747649] |
| Respiratory Physiology & Neurobiology | Surfactant proteins changes after acute hemodynamic improvement in patients with advanced chronic heart failure treated with Levosimendan [Pubmed:29548887] |
| Lung | Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD Exacerbation [Pubmed:29445934] |
| American Journal of Physiology Lung Cellular andMolecular Physiology | Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells [Pubmed: 30211653] |
| Respiratory Research | Culture of human alveolar epithelial type II cells by sprouting [Pubmed: 30340591] |
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