肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)
CD253; TNFSF10; APO2L; Apo2-L; TL2; TRAIL; Tumor Necrosis Factor Ligand Superfamily Member 10; Apo-2 Ligand
- 编号KSA139Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.061ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 5378 ¥ 8964 ¥ 16135 ¥ 31374 ¥ 53784
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特异性
试剂盒中的抗体对肿瘤坏死因子相关凋亡诱导配体(TRAIL)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPA139Hu01 | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA139Hu02 | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA139Hu01 | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)活性蛋白 | Cell culture; Activity Assays. |
PAA139Hu01 | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)多克隆抗体 | WB; IHC; ICC; IP. |
PAA139Hu02 | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)多克隆抗体 | WB; IHC; ICC; IP. |
LAA139Hu71 | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)多克隆抗体(生物素标记) | WB; IHC; ICC. |
LAA139Hu81 | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
MAA139Hu22 | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)单克隆抗体 | WB |
MAA139Hu21 | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)单克隆抗体 | WB; IHC; ICC; IP. |
MAA139Hu23 | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)单克隆抗体 | WB; IHC; ICC; IP. |
LAA139Hu72 | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)单克隆抗体(生物素标记) | WB; IHC; ICC. |
LAA139Hu82 | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)单克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
SEA139Hu | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA139Hu | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA139Hu | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA139Hu01 | 肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
PLOS ONE | Intranasal Administration of Recombinant TRAIL Down-Regulates CXCL-1/KC in an Ovalbumin-Induced Airway Inflammation Murine Model [Pubmed:25506835] |
PLOS ONE | Soluble TRAIL Concentration in Serum Is Elevated in People with Hypercholesterolemia [PubMed: 26633016] |
Scientific Reports | Non-alcoholic fatty liver disease, vascular inflammation and insulin resistance are exacerbated by TRAIL deletion in mice [pubmed:28507343] |
Journal of Molecular Medicine | TRAIL and TRAIL Receptors as Prognostic Markers in Breast Cancer Patients [Pubmed: 31183506] |
life sciences | Nifuroxazide attenuates experimentally-induced hepatic encephalopathy and the associated hyperammonemia and cJNk/caspase-8/TRAIL activation in rats [Pubmed: 32259601] |
Int Immunopharmacol | Cardio-protective impact of gabapentin against doxorubicin-induced myocardial toxicity in rats; emphasis on modulation of inflammatory-apoptotic signaling [Pubmed: 33199237] |
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | Chemo-preventive effect of crocin against experimentally-induced hepatocarcinogenesis via regulation of apoptotic and Nrf2 signaling pathways [Pubmed: 32942000] |
J Biochem Mol Toxicol | Tranilast abrogates cisplatin©\induced testicular and epididymal injuries: An insight into its modulatory impact on apoptosis/proliferation [34047436] |
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