酪氨酸羟化酶(TH)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Tyrosine Hydroxylase (TH)
TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14
- 编号KSB438Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.066ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7182 ¥ 11970 ¥ 21546 ¥ 41895 ¥ 71820
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对酪氨酸羟化酶(TH)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPB438Hu01 | 酪氨酸羟化酶(TH)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB438Hu01 | 酪氨酸羟化酶(TH)多克隆抗体 | WB; IHC; ICC; IP. |
MAB438Hu21 | 酪氨酸羟化酶(TH)单克隆抗体 | WB; IHC; ICC; IP. |
MAB438Hu24 | 酪氨酸羟化酶(TH)单克隆抗体 | WB; IHC; ICC; IP. |
MAB438Hu25 | 酪氨酸羟化酶(TH)单克隆抗体 | WB; IHC; ICC; IP. |
MAB438Hu22 | 酪氨酸羟化酶(TH)单克隆抗体 | WB; IHC; ICC; IP. |
SEB438Hu | 酪氨酸羟化酶(TH)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB438Hu | 酪氨酸羟化酶(TH)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB438Hu01 | 酪氨酸羟化酶(TH)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Mol Neurobiol. | 1, 25-dyhydroxyvitamin D3 Attenuates l-DOPA-Induced Neurotoxicity in Neural Stem Cells [Pubmed:25102940] |
PLOS ONE | Genetic Diagnosis of Two Dopa-Responsive Dystonia Families by Exome Sequencing [Pubmed:25181484] |
biomedicine & pharmacotherapy | Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. [pubmed:28199883] |
Therapeutics and Clinical Risk Management | Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population. [pubmed:27540296] |
Sci Rep. | Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats. [pubmed:28230187] |
Journal of Cellular Biochemistry | Vaccarin administration ameliorates hypertension and cardiovascular remodeling in renovascular hypertensive rats [pubmed:28681939] |
Environmental Science & Technology | Multigenerational disruption of the thyroid endocrine system in marine medaka after a life-cycle exposure to perfluorobutanesulfonate [Pubmed:29565584] |
Molecular Neurobiology | Cilostazol Mediated Nurr1 and Autophagy Enhancement: Neuroprotective Activity in Rat Rotenone PD Model [Pubmed:29429051] |
Chemosphere | Tetrabromobisphenol A caused neurodevelopmental toxicity via disrupting thyroid hormones in zebrafish larvae [Pubmed:29407805] |
Neurospine | The Effect of Recombinant Tyrosine Hydroxylase Expression on the Neurogenic Differentiation Potency of Mesenchymal Stem Cells [Pubmed:29656620] |
Toxicology and Applied Pharmacology | Montelukast attenuates rotenone-induced microglial activation/p38 MAPK expression in rats: Possible role of its antioxidant, anti-inflammatory and antiapoptotic effects [Pubmed: 30222980] |
International Journal Of Cardiology | Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus [Pubmed: 30581101] |
Behavioural Brain Research | Hydroxychloroquine antiparkinsonian potential: Nurr1 modulation versus autophagy inhibition [] |
Environment International | Perfluoropolyether carboxylic acids (novel alternatives to PFOA) impair zebrafish posterior swim bladder development via thyroid hormone disruption [Pubmed: 31733528] |
Environmental Pollution | Embryonic exposure to pentabromobenzene inhibited the inflation of posterior swim bladder in zebrafish larvae [Pubmed: 31935612] |
International Journal of Pharmacology | Nose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's disease [Pubmed: 32084576] |
comparative biochemistry and physiology c-toxicology & pharmacology | Thyroid disruption and developmental toxicity caused by Cd2+ in Schizopygopsis younghusbandi larvae [Pubmed: 32344129] |
International Journal of Stem cell Research & Therapy | Selection of Cells for Parkinson's Disease Cell-Therapy [] |
Ecotoxicol Environ Saf | Effects of SiO2 nanoparticles on the uptake of tetrabromobisphenol A and its impact on the thyroid endocrine system in zebrafish larvae [33385677] |
Nutr Res | Tocotrienols protect differentiated SH-SY5Y human neuroblastoma cells against 6-hydroxydopamine-induced cytotoxicity by ameliorating dopamine biosynthesis and … [Pubmed:35065349] |
Food Chem Toxicol | Omarigliptin attenuates rotenone-induced Parkinson's disease in rats: Possible role of oxidative stress, endoplasmic reticulum stress and immune modulation [Pubmed:35439590] |
Food Chemistry: Molecular Sciences | Unravelling the neuroprotective mechanisms of carotenes in differentiated human neural cells: biochemical and proteomic approaches [Pubmed:35415676] |
Journal of Environmental Sciences | Parental and transgenerational impairments of thyroid endocrine system in zebrafish by 2, 4, 6-tribromophenol [] |
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