波形蛋白(VIM)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Vimentin (VIM)
- 编号KSB040Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围125-8000pg/mL
- 可适灵敏度46pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7182 ¥ 11970 ¥ 21546 ¥ 41895 ¥ 71820
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对波形蛋白(VIM)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB040Hu02 | 波形蛋白(VIM)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB040Hu01 | 波形蛋白(VIM)活性蛋白 | Cell culture; Activity Assays. |
| RPB040Hu01 | 波形蛋白(VIM)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB040Hu02 | 波形蛋白(VIM)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB040Hu01 | 波形蛋白(VIM)多克隆抗体 | WB; IHC; ICC/IF |
| LAB040Hu71 | 波形蛋白(VIM)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB040Hu24 | 波形蛋白(VIM)单克隆抗体 | WB; IHC |
| MAB040Hu25 | 波形蛋白(VIM)单克隆抗体 | WB; IHC; IF |
| MAB040Hu21 | 波形蛋白(VIM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB040Hu23 | 波形蛋白(VIM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB040Hu27 | 波形蛋白(VIM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB040Hu22 | 波形蛋白(VIM)单克隆抗体 | WB; IHC; IF |
| FAB040Hu02 | 抗波形蛋白(VIM)单克隆抗体 | Flow cytometry. |
| FAB040Hu06 | 抗波形蛋白(VIM)单克隆抗体 | Flow cytometry. |
| FAB040Hu04 | 抗波形蛋白(VIM)单克隆抗体 | Flow cytometry. |
| FAB040Hu08 | 抗波形蛋白(VIM)单克隆抗体 | Flow cytometry. |
| SEB040Hu | 波形蛋白(VIM)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB040Hu | 波形蛋白(VIM)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB040Hu01 | 波形蛋白(VIM)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Cancer Epidemiology, Biomarkers & Prevention | Aberrant Vimentin Methylation Is Characteristic of Breast Cancer [pubmed:22315367] |
| Scientific Reports | Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome andendothelial function estimates. [pubmed:27898103] |
| Journal of Proteomics | HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin [Pubmed:29477555] |
| Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy [Pubmed: 31511328] |
| American Journal of Transplantation | Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allografts [Pubmed: 31729155] |
| Lung | Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development? [Pubmed: 32088750] |
| Cell Biol Toxicol | ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer [33566221] |
| CELL BIOLOGY AND TOXICOLOGY | Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence [33758996] |
| Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women [] | |
| Advanced glycation end-products associate with podocytopathy in type II diabetic patients [] | |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … [] |
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