玻连蛋白(VTN)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Vitronectin (VTN)
V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein
- 编号KSB041Ra01
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Plasma.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围7.81-500ng/mL
- 可适灵敏度3.7ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对玻连蛋白(VTN)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPB041Ra01 | 玻连蛋白(VTN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB041Ra01 | 玻连蛋白(VTN)活性蛋白 | Cell culture; Activity Assays. |
PAB041Ra01 | 玻连蛋白(VTN)多克隆抗体 | WB; IHC; ICC; IP. |
MAB041Ra21 | 玻连蛋白(VTN)单克隆抗体 | WB; IHC; ICC; IP. |
SEB041Ra | 玻连蛋白(VTN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB041Ra | 玻连蛋白(VTN)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB041Ra01 | 玻连蛋白(VTN)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Biomedical Engineering and Informatics | Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer [IEEE: 6098563] |
PLoS ONE | Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance [PubMed: PMC3754973] |
Liver International | Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 2-D DIGE [Pubmed: 23944848] |
Analytical Biochemistry | A multianalytical approach to investigate the effect of nanofiltration on plasma-derived factor IX clinical lots [Pubmed:29154788] |
Journal of Materials Chemistry B | Serum protein adsorption profile on BCP ceramic and influence of the elevated adsorption of adhesive proteins on the behaviour of MSCs [Doi: 10.1039/C8TB02283F ] |
JOURNAL OF NEPHROLOGY | Urinary vitronectin identifies patients with high levels of fibrosis in kidney grafts [33275196] |
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