非组织特异性碱性磷酸酶(ALPL)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Alkaline Phosphatase, Tissue-nonspecific (ALPL)
BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase,Tissue-Nonspecific Isozyme; Alkaline Phosphatase, Liver/Bone/Kidney
- 编号SEB091Rb
- 物种Oryctolagus cuniculus (Rabbit，兔) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.312-20ng/mL
- 灵敏度最小可检测剂量小于等于0.122ng/mL.
- 样本类型tissue homogenates, cell lysates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3032 ¥ 4332 ¥ 19494 ¥ 36822 ¥ 303240
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测非组织特异性碱性磷酸酶(ALPL)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将非组织特异性碱性磷酸酶(ALPL)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的非组织特异性碱性磷酸酶(ALPL)与连接于固相载体上的抗体结合，然后加入生物素化的非组织特异性碱性磷酸酶(ALPL)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的非组织特异性碱性磷酸酶(ALPL)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Oryctolagus cuniculus (Rabbit，兔) | 应用(仅供研究使用，不用于临床诊断！) |
| SEB091Rb | 非组织特异性碱性磷酸酶(ALPL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB091Rb | 非组织特异性碱性磷酸酶(ALPL)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Journal of Materials Science: Materials in Medicine | Osteopenic bone cell response to strontium-substituted hydroxyapatite [SpringerLink: n8233337w171v451] |
| Osteoporosis International | Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-κB activation [PubMed: 23400250] |
| Journal of Applied Toxicology | Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice [Wiley: Source] |
| Acta Pharmacologica Sinica | The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model [Pubmed:25544359] |
| Biomedical Materials | Enhanced differentiation of osteoblastic cells on novel chitosan/β-1, 3-glucan/bioceramic scaffolds for bone tissue regeneration [Pubmed:25586067] |
| Pharmacology | Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy [PubMed: 25871861] |
| J Biomed Mater Res A. | New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization. [Pubmed:27239050] |
| J Bone Miner Metab | MiR-142-5p promotes bone repair by maintaining osteoblast activity [Pubmed:27085967] |
| Journal of Biomedical Materials Research | New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological … [doi:10.1002] |
| Journal of Bone and Mineral Metabolism | MiR‑142‑5p promotes bone repair by maintaining osteoblast activity [pubmed:27085967] |
| Scientific Reports | Hydrolysis of Extracellular Pyrophosphate increases in post-hemodialysis plasma [Pubmed:30038263] |
| International Journal of Molecular Sciences | Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In Vivo [Pubmed:29562730] |
| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457] |
| Experimental and Therapeutic Medicine | Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice [10.3892:etm.2018.6353] |
| American Journal of Translational Research | Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9 [] |
| The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro [] | |
| International Journal of Biomaterials | Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect [34104195] |
