抗突触核蛋白α抗体(Anti-SNCa)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Anti-Synuclein Alpha Antibody (Anti-SNCa)
SNC-A; aSYN; PD1; PARK1; PARK4; NACP; Non-A Beta Component Of Alzheimer's Disease Amyloid Precursor Protein; Non-A4 component of amyloid precursor
- 编号AEB222Hu
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 实验方法间接法
- 反应时长2h, 30min
- 检测范围3.12-200ng/mL
- 灵敏度最小可检测剂量小于等于1.14ng/mL.
- 样本类型Serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
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特异性
本试剂盒用于检测抗突触核蛋白α抗体(Anti-SNCa),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的抗突触核蛋白α抗体(Anti-SNCa)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 85-103 | 93 |
EDTA plasma(n=5) | 99-105 | 102 |
heparin plasma(n=5) | 90-97 | 94 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的抗突触核蛋白α抗体(Anti-SNCa),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中抗突触核蛋白α抗体(Anti-SNCa)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 90-97% | 89-96% | 96-103% | 98-105% |
EDTA plasma(n=5) | 79-103% | 80-90% | 88-101% | 83-99% |
heparin plasma(n=5) | 82-89% | 82-91% | 86-95% | 80-89% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板5次;
5. 加TMB底物90µL,37°C孵育10-20分钟;
6. 加终止液50µL,立即450nm读数。
实验原理
将抗原包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的抗突触核蛋白α抗体(Anti-SNCa)与连接于固相载体上的抗原结合,然后加入HRP标记的二抗,彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗突触核蛋白α抗体(Anti-SNCa)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPB222Hu01 | 突触核蛋白α(SNCa)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB222Hu01 | 突触核蛋白α(SNCa)多克隆抗体 | WB; IHC; ICC; IP. |
LAB222Hu71 | 突触核蛋白α(SNCa)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAB222Hu22 | 突触核蛋白α(SNCa)单克隆抗体 | WB; IHC; ICC; IP. |
SEB222Hu | 突触核蛋白α(SNCa)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
AEB222Hu | 抗突触核蛋白α抗体(Anti-SNCa)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
参考文献
杂志 | 参考文献 |
The Journal of Clinical Investigation | Cerebrospinal fluid–based kinetic biomarkers of axonal transport in monitoring neurodegeneration [PubMed: PMC3428100] |
Neurobiology of Disease | Lysosomal dysfunction increases exosome-mediated alpha-synuclein release and transmission [PubMed: PMC3107939] |
Nature Communications | Parkinson's disease induced pluripotent stem cells with triplication of the α-synuclein locus [PubMed: PMC3265381] |
Provisional chapter | Mutations of PARK Genes and Alpha-Synuclein and Parkin Concentrations in Parkinson’s Disease [Intechopen:Source] |
Nerodegeneration | Apolipoprotein Eε4: A Biomarker for Executive Dysfunction among Parkinson's Disease Patients with Mild Cognitive Impairment [pubmed:29326545] |
Experimental gerontology | The anti-aging protein klotho alleviates injury of nigrostriatal dopaminergic pathway in 6-hydroxydopamine rat model of Parkinson's disease: Involvement of PKA/CaMKII/CREB signaling. [pubmed:29107062] |
Neurobiology of Disease | Development and biochemical characterization of a mouse model of Parkinson's disease bearing defective glucocerebrosidase activity [Pubmed: 30521842] |
molecular and cellular biochemistry | Potential therapeutic effects of antagonizing adenosine A2A receptor, curcumin and niacin in rotenone-induced Parkinson's disease mice model [Pubmed: 31820278] |
International Journal of Pharmacology | Nose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's disease [Pubmed: 32084576] |
EBioMedicine | Anti-α-synuclein ASO delivered to monoamine neurons prevents α-synuclein accumulation in a Parkinson's disease-like mouse model and in monkeys [Pubmed: 32810825] |