脑钠素(BNP)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Brain Natriuretic Peptide (BNP)
GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32
- 编号SEA541Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围31.2-2,000pg/mL
- 灵敏度最小可检测剂量小于等于14.1pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2793 ¥ 3990 ¥ 17955 ¥ 33915 ¥ 279300
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测脑钠素(BNP)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的脑钠素(BNP)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 80-92 | 80 |
| EDTA plasma(n=5) | 97-105 | 102 |
| heparin plasma(n=5) | 82-89 | 86 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的脑钠素(BNP)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中脑钠素(BNP)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-101% | 88-95% | 94-105% | 87-103% |
| EDTA plasma(n=5) | 92-101% | 96-105% | 85-97% | 86-101% |
| heparin plasma(n=5) | 90-104% | 98-105% | 93-105% | 98-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将脑钠素(BNP)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的脑钠素(BNP)与连接于固相载体上的抗体结合，然后加入生物素化的脑钠素(BNP)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的脑钠素(BNP)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA541Hu01 | 脑钠素(BNP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| SPA541Hu01 | 脑钠素(BNP)合成多肽 | Immunogen; Blocking Peptide. |
| CPA541Hu21 | 脑钠素(BNP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA541Hu11 | 脑钠素(BNP)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA541Hu22 | 脑钠素(BNP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA541Hu23 | 脑钠素(BNP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA541Hu24 | 脑钠素(BNP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA541Hu01 | 脑钠素(BNP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA541Hu02 | 脑钠素(BNP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA541Hu07 | 脑钠素(BNP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA541Hu08 | 脑钠素(BNP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA541Hu09 | 脑钠素(BNP)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA541Hu81 | 脑钠素(BNP)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA541Hu71 | 脑钠素(BNP)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA541Hu27 | 脑钠素(BNP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA541Hu29 | 脑钠素(BNP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA541Hu22 | 脑钠素(BNP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA541Hu26 | 脑钠素(BNP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA541Hu28 | 脑钠素(BNP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA541Hu21 | 脑钠素(BNP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA541Hu23 | 脑钠素(BNP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA541Hu24 | 脑钠素(BNP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA541Hu25 | 脑钠素(BNP)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA541Hu | 脑钠素(BNP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA541Hu | 脑钠素(BNP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA541Hu | 脑钠素(BNP)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA541Hu | 脑钠素(BNP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA541Hu11 | 脑钠素(BNP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Heart and Circulatory Physiology | B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness [APS: source] |
| PLoS One | Renalase deficiency in heart failure model of rats_a potential mechanism underlying circulating norepinephrine accumulation. [PubMed: 21297953] |
| International Journal of Toxicology | Cardiac Troponin T and NT-proBNP as Biomarkers of Early Myocardial Damage in Amitriptyline-Induced Cardiovascular Toxicity in Rats [Pubmed: 24055824] |
| JACC | ELEVATED ST2 SERUM LEVELS A BIOMARKER FOR CARDIOMYOPATHY IN DUCHENNE MUSCULAR DYSTROPHY [Onlinejacc:Source] |
| J Urol.? | Why does Adenotonsillectomy Not Correct Enuresis in All Children with Sleep Disordered Breathing? [Pubmed:24679871] |
| Life Sci | Cardioprotective effects of baicalein on heart failure via modulation of Ca2 [PubMed: 26706290] |
| Vlaams Diergeneeskundig Tijdschrift | Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study [Publication: 5950761] |
| Physiological Research | Renal Sympathetic Denervation Improves Cardiac Dysfunction [PubMed: 25804096] |
| Life Sci | Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro [Pubmed:26706290] |
| J Ethnopharmacol | Danhong injection attenuates isoproterenol-induced cardiac hypertrophy by reCavia (Guinea pig )lating p38 and NF-κb pathway [Pubmed:26970569] |
| Echocardiography. | Improvement in the prediction of exercise-induced elevation of left ventricular filling pressure in patients with normal left ventricular ejection fraction. [pubmed:27779336] |
| SpringerPlus | The effect of long-term amiodarone administration on myocardial fibrosis and evolution of left ventricular remodeling in a porcine model of ischemic … [pubmed:27652141] |
| Heart Lung Circ. | Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure. [pubmed:27555054] |
| Clinica Chimica Acta | Plasma concentration of fibronectin is decreased in patients with hypertrophic cardiomyopathy [pubmed:27693530] |
| Scientific Reports | Paracrine cross-talk between skeletal muscle and macrophages in exercise by PGC-1α-controlled BNP. [pubmed:28091624] |
| Molecular Medicine Report | Intermedin improves cardiac function and sympathetic neural remodeling in a rat model of post myocardial infarction heart failure [pubmed:28627670] |
| PLoS One | Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy [pubmed:29240788] |
| Acta Physiologica | Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload [pubmed:27575955] |
| International Immunopharmacology | Metformin attenuated endotoxin-induced acute myocarditis via activating AMPK [pubmed:28410530] |
| Drug Delivery | Ginsenoside Rg3 micelles mitigate doxorubicin-induced cardiotoxicity and enhance its anticancer efficacy. [pubmed:29063791] |
| Science of the Total Environment | Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and [Pubmed:29727991] |
| Scientific Reports | An essential role for Wnt/β-catenin signaling in mediating hypertensive heart disease [Pubmed:29895976] |
| International Journal of Cardiology | Cardioprotective effect of substance P in a porcine model of acute myocardial infarction [Pubmed:29884290] |
| International Journal of Cardiology | High-intensity interval training enhances mitochondrial bioenergetics of platelets in patients with heart failure [Pubmed:30072155] |
| Evidence-based Complementary and Alternative Medicine | Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects [Pubmed:29977313] |
| Molecular Medicine Reports | Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats [Pubmed:29956781] |
| Pediatric Cardiology | Determinants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart Disease [Pubmed:29392348] |
| BMC Pharmacology and Toxicology | Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis [Pubmed:29321061] |
| 中国病理生理杂志 | 和肽素水平对大鼠心肾综合征的早期预测价值 [filename:ZBLS201802013] |
| FASEB Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922] |
| Toxicology Mechanisms and Methods | Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats [Pubmed: 30426860] |
| Van Veterinary Journal | Van Veterinary Journal [] |
| Kidney International | Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome [Pubmed: 30770217] |
| Journal of Cellular Physiology | Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytes [Pubmed: 30982981] |
| Cardiology | Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart Failure [Pubmed: 31466059] |
| BIOMEDICINE & PHARMACOTHERAPY | Pharmacodynamic and pharmacokinetic effects of S086, a novel angiotensin receptor neprilysin inhibitor [Pubmed: 32570118] |
| biochimica et biophysica acta-molecular basis of disease | Empagliflozin improves left ventricular diastolic function of db/db mice [Pubmed: 32353614] |
| Dapagliflozin: a Sodium-glucose Cotransporter 2 Inhibitor, Attenuates Angiotensin II-induced Cardiac Fibrotic Remodeling by Regulating TGF¦Â1/Smad ¡ [34116674] | |
| Radiation-induced heart energy metabolism dysfunction leads to heart fibrosis [] | |
| Oxid Med Cell Longev | κ Opioid Receptor Agonist Inhibits Myocardial Injury in Heart Failure Rats through Activating Nrf2/HO-1 Pathway and Regulating Ca2+-SERCA2a [34373768] |
| Mol Med Rep | Radiation‑induced dysfunction of energy metabolism in the heart results in the fibrosis of cardiac tissues [34633055] |
| viruses | Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model [34452454] |
| Peptides | Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stress [Pubmed:35218795] |
留言咨询