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趋化素(CHEM)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Chemerin (CHEM)

TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2

  • 趋化素(CHEM)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 趋化素(CHEM)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 趋化素(CHEM)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA945Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测趋化素(CHEM),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的趋化素(CHEM)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-94 89
EDTA plasma(n=5) 81-95 88
heparin plasma(n=5) 89-102 95

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的趋化素(CHEM),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中趋化素(CHEM)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 86-104% 86-102% 95-105%
EDTA plasma(n=5) 83-97% 95-102% 80-90% 83-97%
heparin plasma(n=5) 89-96% 87-103% 86-101% 79-97%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将趋化素(CHEM)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的趋化素(CHEM)与连接于固相载体上的抗体结合,然后加入生物素化的趋化素(CHEM)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的趋化素(CHEM)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA945Mu01 趋化素(CHEM)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA945Mu02 趋化素(CHEM)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA945Mu02 趋化素(CHEM)多克隆抗体 WB; IHC; ICC; IP.
PAA945Mu01 趋化素(CHEM)多克隆抗体 WB,IHC
SEA945Mu 趋化素(CHEM)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA945Mu 趋化素(CHEM)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA945Mu01 趋化素(CHEM)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Scandinavian Journal of Gastroenterology Serum chemerin and vaspin in non-alcoholic fatty liver disease [PubMed: 20095887]
Gastroenterology & Hepatology Chemerin, vaspin and insulin resistance in chronic hepatitis C [Wiley: source]
Placenta Expression and regulation of chemerin during rat pregnancy [ScienceDirect: S0143400412000902]
Acta Pharmacologica Sinica Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and rats [Nature: 201211]
RAED dergisi Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnci [Raeddergisi: Source]
Journal of Cellular and Molecular Medicine Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patients [Pubmed: 24702860]
Clinical Endocrinology Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjects [Pubmed: 24303851]
Oral Dis. Impact of OSA on Biological Markers in Morbid Obesity and Metabolic Syndrome [Pubmed:24106991]
Endocrine Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia [Pubmed:24840719]
The Clinical Respiratory Journal Relationship between plasma chemerin levels and disease severity in COPD patients [Pubmed:24865134]
Gynecol Endocrinol. Elevated serum chemerin in Chinese women with hyperandrogenic PCOS [Pubmed:24927079]
Endocrine. Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats. [Pubmed:25471281]
Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126]
Dermatologica Sinica Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgaris [Sciencedirect:S1027811714000470]
Dis Markers Serum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control Study [PubMed: 26170530]
Med Sci Monit Association of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese Population [PubMed: 26471865]
Endocrine Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats [PubMed: 25471281]
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytes [Pubmed:26721419]
Medicine (Baltimore). Association of chemerin levels and bone mineral density in Chinese obese postmenopausalwomen. [pubmed:27583869]
Oxidative Medicine and Cellular Longevity Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague … [Pubmed:29849871]
Current Eye Research Chemerin is associated with diabetic retinopathy in type 2 diabetes [Pubmed:25848840]
Journal of Oral Rehabilitation Expression of chemerin in the synovial fluid of patients with temporomandibular joint disorders [Pubmed:29331039]
International Journal of Cancer Epigenetic regulation of Amphiregulin and Epiregulin in colorectal cancer [Pubmed: 30252132]
Experimental Gerontology The decline of whole-body glucose metabolism in ovariectomized rats [Pubmed: 30292771]
Pancreatology Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-κB signal [Doi: 10.1016/j.pan.2019.02.005]
Hypertens Pregnancy Protective effect of vitamin D supplementation in a rat modal of preeclampsia: a possible implication of chemerin [Pubmed: 30922121]
Pancreatology Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-kB signal [Pubmed: 30833212]
Digestion Association between High Levels of Circulating Chemerin and Colorectal Adenoma in Men [Pubmed: 31269491]
Vitamin D Supplementation Reduces Serum Chemerin Level in Gestational Diabetes Mellitus Rat Model []
Aging Medicine Plasma levels of adiponectin and chemerin are associated with early stage of atherosclerosis in older adults with type 2 diabetes mellitus []
Eur J Nutr The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren [33575861]
Eur J Pediatr Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren [33834274]
Indian Journal of Forensic Medicine & Toxicology Altered Serum Marker of Adipokines Profile in Breast Cancer Women []
Int J Womens Health Association of High Serum Chemerin with Bone Mineral Density Loss and Osteoporotic Fracture in Elderly Chinese Women [Pubmed:35140527]
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