细胞色素C(CYCS)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Cytochrome C (CYCS)
Cyt-C; HCS; CYC; CytC
- 编号SEA594Mi
- 物种Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围1.56-100ng/mL
- 灵敏度最小可检测剂量小于等于0.55ng/mL.
- 样本类型Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2722 ¥ 3888 ¥ 17496 ¥ 33048 ¥ 272160
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特异性
本试剂盒对检测细胞色素C(CYCS)具有较高的灵敏度。
经检测人、小鼠、大鼠之间有100%的交叉反映。
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。
实验原理
将细胞色素C(CYCS)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的细胞色素C(CYCS)与连接于固相载体上的抗体结合,然后加入生物素化的细胞色素C(CYCS)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的细胞色素C(CYCS)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA594Mi01 | 细胞色素C(CYCS)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA594Mi01 | 细胞色素C(CYCS)多克隆抗体 | WB; IHC; ICC; IP. |
CAA594Mi22 | 抗细胞色素C(CYCS)单克隆抗体 | Loading Control of WB |
SEA594Mi | 细胞色素C(CYCS)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA594Mi | 细胞色素C(CYCS)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA594Mi01 | 细胞色素C(CYCS)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
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