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多巴胺(DA)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Dopamine (DA)

2-(3,4-Dihydroxyphenyl)ethylamine; 3,4-Dihydroxyphenethylamine; 3-Hydroxytyramine; Intropin; Revivan; Oxytyramine; Dopamine Hydrochloride

  • 多巴胺(DA)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 多巴胺(DA)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 多巴胺(DA)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA851Ge.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测多巴胺(DA),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的多巴胺(DA)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 95-102 99
EDTA plasma(n=5) 86-93 89
heparin plasma(n=5) 90-99 94

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的多巴胺(DA),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中多巴胺(DA)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 94-103% 94-105% 97-104% 85-104%
EDTA plasma(n=5) 78-102% 92-105% 80-99% 79-101%
heparin plasma(n=5) 91-99% 85-103% 78-93% 80-101%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将多巴胺(DA)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPA851Ge41 多巴胺(DA)人血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA851Ge11 多巴胺(DA)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA851Ge21 多巴胺(DA)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA851Ge01 多巴胺(DA)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA851Ge71 多巴胺(DA)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA851Ge81 多巴胺(DA)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA851Ge21 多巴胺(DA)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MEA851Ge 多巴胺(DA)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA851Ge 多巴胺(DA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA851Ge 多巴胺(DA)检测试剂盒(酶联免疫吸附试验法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA851Ge 多巴胺(DA)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA851Ge 多巴胺(DA)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA851Ge11 多巴胺(DA)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Renal Failure Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients [Pubmed: 23530612]
Molecular Neurobiology Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations [Pubmed:25502462]
Molecular Neurobiology Bone Marrow-Derived Endothelial Progenitor Cells Protect Against Scopolamine-Induced Alzheimer-Like Pathological Aberrations [Pubmed:25526861]
Cardiology. Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adults [Pubmed:25824645]
Eur J Pharmacol Nalbuphine could decrease the rewarding effect induced by tramadol in mice while enhancing its antinociceptive activity [PubMed: 25843409]
Neuropsychiatr Dis Treat Catha edulis chewing effects on treatment of paranoid schizophrenic patients [PubMed: 25926735]
Journal of Neurochemistry Neuroprotective effects of vildagliptin in rat rotenone Parkinson's disease model: role of RAGE‐NFκB and Nrf2‐antioxidant signaling pathways [PubMed: 25752913]
Beni-Suef University Journal of Basic and Applied Sciences Modulatory effect of cilostazol on tramadol-induced behavioral and neurochemical alterations in rats challenged across the forced swim despair test [science:S2314853516300166]
Neuroscience Imipramine and amitriptyline ameliorate the rotenone model of Parkinson's disease in rats [pubmed:27365173]
Metab Brain Dis. Neuroprotective effect of Cucumis melo Var. flexuosus leaf extract on the brains of rats with streptozotocin-induced diabetes. [pubmed:27488111]
Nutrition Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in rats [S0899900716302350]
Nutrition Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats [pubmed:28241996]
Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Rats [article_1486_c0fa9f8afe5f70b407e7195bcfd55c70]
Behavioural Brain Research High Fat Diet feeding induced insulin resistance exacerbates 6-OHDA mediated neurotoxicity and behavioral abnormalities in rats [Pubmed:29842916]
Toxicology Reports Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor … [Pubmed:29854627]
Molecular and Cellular Biochemistry Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes [Pubmed:28948423]
Physiology & Behavior  Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll … [Pubmed:29410018]
Behavioural Brain Research Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats [Pubmed: 30423388]
Indian Journal of Ophthalmology Dopamine levels in human tear fluid [Pubmed: 30574889]
Journal of Taibah University for Science Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles []
Metabolic Brain Disease Soya bean rich diet is associated with adult male rat aggressive behavior: relation to RF amide-related peptide 3-aromatase-neuroestrogen pathway in the brain [Pubmed: 31134480]
Functional activity of p-glycoprotein in blood-brain barrier during experimental par-kinson's syndrome []
Neurotoxicity of Mn3O4 nanoparticles: Apoptosis and dopaminergic neurons damage pathway [Pubmed: 31740235]
Neurochemistry International Polydatin protects SH-SY5Y in models of Parkinson's disease by promoting Atg5-mediated but parkin-independent autophagy [Pubmed: 31926197]
Neurochemistry International Iron overload induced by IRP2 gene knockout aggravates symptoms of Parkinson's disease [Pubmed: 31904393]
Journal of neural transmission (Vienna, Austria : 1996) Neurotransmitter system aberrations in patients with drug addiction [Pubmed: 32804296]
Acupunct Med Electroacupuncture alleviates intestinal inflammation and barrier dysfunction by activating dopamine in a rat model of intestinal ischaemia [Pubmed: 32517478]
Effect of metformin and indole-3-carbinol on a rat model of Parkinson's disease induced by 6-hydroxydopamine []
South African Journal of Botany Neuroprotective effects of pulicaria undulata essential oil in rotenone model of parkinson's disease in rats: Insights into its anti-inflammatory and anti-oxidant effects []
ACTA PHARMACOLOGICA SINICA The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice [33303990]
Neural Regeneration Research Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6?¡­ [33433498]
International Journal of?Nanomedicine Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡­ [33762821]
CRYOBIOLOGY O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress [33651993]
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA A pair of dopamine neurons mediate chronic stress signals to induce learning deficit in Drosophila melanogaster [34654742]
ACS Chem Neurosci Neuroprotective Effects of Trehalose and Sodium Butyrate on Preformed Fibrillar Form of α-Synuclein-Induced Rat Model of Parkinson's Disease [34197084]
Exp Brain Res Effects of early life stress during stress hyporesponsive period (SHRP) on anxiety and curiosity in adolescent rats [Pubmed:35141770]
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