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白介素13(IL13)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Interleukin 13 (IL13)

ALRH; BHR1; P600; IL-13; NC30

  • 白介素13(IL13)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 白介素13(IL13)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 白介素13(IL13)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA060Eq.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测白介素13(IL13)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的白介素13(IL13)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 84-91 88
EDTA plasma(n=5) 87-101 95
heparin plasma(n=5) 94-105 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的白介素13(IL13)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中白介素13(IL13)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 90-97% 78-93% 95-103% 80-96%
EDTA plasma(n=5) 81-101% 90-97% 83-97% 96-103%
heparin plasma(n=5) 87-103% 95-104% 80-103% 78-104%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将白介素13(IL13)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的白介素13(IL13)与连接于固相载体上的抗体结合，然后加入生物素化的白介素13(IL13)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白介素13(IL13)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Equus caballus; Equine (Horse，马) 应用(仅供研究使用，不用于临床诊断！)
RPA060Eq01 白介素13(IL13)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA060Eq01 白介素13(IL13)多克隆抗体 WB; IHC; ICC; IP.
MAA060Eq21 白介素13(IL13)单克隆抗体 WB; IHC; ICC; IP.
SEA060Eq 白介素13(IL13)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA060Eq 白介素13(IL13)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Critical Care Medicine Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage [PubMed: PMC3383056]
Int J Clin Exp Pathol Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation [PubMed: 26722390]
Advances in Experimental Medicine and Biology  Antiinflammatory Effect of N-Acetylcysteine Combined with Exogenous Surfactant in Meconium-Induced Lung Injury [Pubmed:27283193]
Molecular Immunology Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by reCavia (Guinea pig )lating caveolin-1 through TLR2/MyD88/NF-ŚĘB in murine model of asthma [Pubmed:27289030]
Journal of Agricultural Biotechnology Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells [10610]
BMC Veterinary Research Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis [pubmed:27553600]
Molecular Immunology Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma [pubmed:27289030]
Physiological Research Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome. [pubmed:28006947]
Gut Pathog. Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847]
Veterinary Immunology and Immunopathology Cytokine levels in colostrum and in foals' serum pre- and post-suckling [pubmed:28242000]
Asian Pacific Society of Respirology Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397]
Journal of Autoimmunity Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice [10.1016:j.jaut.2018.07.003]
bulletin of experimental biology and medicine Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin [Pubmed: 31183654]
life sciences Melatonin biosynthesis restored by CpG oligodeoxynucleotides attenuates allergic airway inflammation via regulating NLRP3 inflammasome [Pubmed: 31738882]
Frontiers in Immunology TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway Inflammation [Pubmed: 32117301]
Parasites & Vectors Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism … [Pubmed: 32680553]
Biomedicine & Pharmacotherapy Qingfei oral liquid alleviates airway hyperresponsiveness and mucus hypersecretion via TRPV1 signaling in RSV-infected asthmatic mice [Pubmed: 32521453]
Journal of Traditional Chinese Medical Sciences Mahuang Xixin Fuzi Decoction protects against ovalbumin-induced allergic rhinitis by inhibiting type 2 innate lymphoid cells in mice []
Traditional Medicine Research Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection … []
Applied materials today Tanshinone IIA-loaded aligned microfibers facilitate stem cell recruitment and capillary formation by inducing M2 macrophage polarization []
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota []
Biomed Pharmacother Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice [33706133]
BIOMATERIALS Aligned microfiber-induced macrophage polarization to guide schwann-cell-enabled peripheral nerve regeneration [33813259]
PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies [33999928]
Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS []
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