成纤维细胞生长因子23(FGF23)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Fibroblast Growth Factor 23 (FGF23)
ADHR; HYPF; HPDR2; PHPTC; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor
- 编号SEA746Ra
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围15.6-1000pg/mL
- 灵敏度最小可检测剂量小于等于5.7pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3032 ¥ 4332 ¥ 19494 ¥ 36822 ¥ 303240
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测成纤维细胞生长因子23(FGF23)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的成纤维细胞生长因子23(FGF23)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 86-101 | 96 |
| EDTA plasma(n=5) | 96-104 | 101 |
| heparin plasma(n=5) | 86-101 | 93 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的成纤维细胞生长因子23(FGF23)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中成纤维细胞生长因子23(FGF23)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-102% | 93-101% | 93-101% | 82-103% |
| EDTA plasma(n=5) | 91-101% | 80-90% | 94-102% | 98-105% |
| heparin plasma(n=5) | 78-104% | 93-105% | 98-105% | 98-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将成纤维细胞生长因子23(FGF23)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的成纤维细胞生长因子23(FGF23)与连接于固相载体上的抗体结合，然后加入生物素化的成纤维细胞生长因子23(FGF23)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的成纤维细胞生长因子23(FGF23)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA746Ra01 | 成纤维细胞生长因子23(FGF23)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA746Ra01 | 成纤维细胞生长因子23(FGF23)活性蛋白 | Cell culture; Activity Assays. |
| PAA746Ra01 | 成纤维细胞生长因子23(FGF23)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA746Ra21 | 成纤维细胞生长因子23(FGF23)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA746Ra | 成纤维细胞生长因子23(FGF23)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA746Ra | 成纤维细胞生长因子23(FGF23)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA746Ra | 成纤维细胞生长因子23(FGF23)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA746Ra11 | 成纤维细胞生长因子23(FGF23)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of international medicine | Prognostic impact of renal function in precapillary pulmonary hypertension [Pubmed: 24011362] |
| BMC Nephrology | FGF-23 associated with the progression of coronary artery calcification in hemodialysis patients [Pubmed: 24180481] |
| Journal of Clinical Densitometry | Lower Fibroblast Growth Factor 23 Levels in Young Adults With Crohn Disease as a Possible Secondary Compensatory Effect on the Disturbance of Bone and Mineral Metabolism [Pubmed: 23623649] |
| J Intern Med. | Prognostic impact of renal function in precapillary pulmonary hypertension. [Pubmed:24011362] |
| Journal of Bone and Mineral Research | Adverse effects of osteocytic constitutive activation of ?-catenin on bone strength and bone growth [Pubmed:25639729] |
| Nefrologia | Fibroblast growth factor is associated to left ventricular mass index, anemia and low values of transferrin saturation El factor de crecimiento fibroblástico está asociado con el índice de masa ventricular izquierda, anemia y niveles bajos de saturación de la transferrina [PubMed: 26394828] |
| Medicine (Baltimore) | An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370] |
| Ann Biol Clin (Paris) | Les biomarqueurs des calcifications vasculaires: quelles limites analytiques pour leur transfert de la recherche bioclinique à la pratique? [PubMed: 26069069] |
| Scandinavian Journal of Gastroenterology | High-dose fast infusion of parenteral iron isomaltoside is efficacious in inflammatory bowel disease patients with iron-deficiency anaemia without profound changes in phosphate or fibroblast growth factor 23 [Pubmed:27326766] |
| PLOS ONE | Prostaglandin-E2 Mediated Increase in Calcium and Phosphate Excretion in a Mouse Model ofDistal Nephron Salt Wasting. [pubmed:27442254] |
| Journal of Laboratory Diagnostics | Evaluation of concentration fibroblast growth factor FGF-23 in hemodialysed patients and after kidney transplantation [publication:312592751] |
| Journal of Integrative Nephrology & Andrology | Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patients [issn:2394-2916] |
| Journal of Renal Nutrition | ADAM17, a New Player in the Pathogenesis of Chronic Kidney Disease-Mineral and Bone Disorder. [pubmed:29056164] |
| Experimental Biology and Medicine(Maywood) | Effect of cross-linked chitosan iron (III) on vascular calcification in uremic rats [Pubmed:29763365] |
| Psychiatry Research | Cerebrospinal fluid FGF23 levels correlate with a measure of impulsivity [Pubmed:29677623] |
| BMC Pediatrics | Serum and urine FGF23 and IGFBP-7 for the prediction of acute kidney injury in critically ill children [Pubmed:29907141] |
| Medical Science Monitor | Effect of Salt Intervention on Serum Levels of Fibroblast Growth Factor 23 (FGF23) in Chinese Adults: An Intervention Study [Pubmed:29608553] |
| International Journal of Chronic Obstructive Pulmonary Disease | Disconnection of pulmonary and systemic arterial stiffness in COPD [Pubmed:29881265] |
| Saudi journal of kidney diseases and transplantation | Prevalence of cardiac arrhythmia and risk factors in chronic kidney disease patients [Pubmed:29970732] |
| Nephrology Dialysis Transplantation | High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney disease [Pubmed: 30189026] |
| BioFactors | Alteration in serum concentrations of FGF19, FGF21, and FGF23 in patients with urothelial carcinoma [Pubmed: 30334297] |
| Biological Trace Element Research | Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients [Pubmed: 30847765] |
| Clin Implant Dent Relat Res. | Evaluation of FGF‐23 and 25(OH)D3 levels in peri‐implant sulcus fluid in peri‐implant health and diseases [Pubmed: 31407857] |
| JACC-Cardiovascular Imaging | PREVALANCE AND PROGRESSION OF CARDIOVASCULAR CALCIFICATIONS IN HAEMODIALYSIS PATIENTS [Pubmed: 28797410] |
| Saudi J Kidney Dis Transpl | Serum fibroblast growth factor 23 levels do not correlate with carotid intima-media thickness in patients with chronic kidney disease [Pubmed: 31696838] |
| Biomedicine & Pharmacotherapy | MiRNA-192-5p attenuates airway remodeling and autophagy in asthma by targeting MMP-16 and ATG7 [Pubmed: 31918268] |
| Is Serum FGF-23 Associated with Subclinic Atherosclerosis in Patients with AA Amyloidosis? [] | |
| THE RELATIONSHIP BETWEEN ARTERIAL STIFNESS AND 25-(OH) VITAMIN D, FGF 23 IN MAINTENANCE HEMODIALYSIS PATIENTS [] | |
| THE RELATIONSHIP BETWEEN ARTERIAL STIFFNESS AND 25-(OH) VITAMIN D, FGF 23 IN MAINTENANCE HEMODIALYSIS PATIENTS KRON?K ¡ [] | |
| Calcif Tissue Int | The Measurement and Interpretation of Fibroblast Growth Factor 23 (FGF23) Concentrations [Pubmed:35665817] |
| European Journal of Medical Genetics | Genetic and clinical profile of patients with hypophosphatemic rickets [Pubmed:35738466] |
| African Health Sciences | Fibroblast Growth Factor 23 (FGF 23) and intact parathyroid hormone (iPTH) as markers of mineral bone disease among Nigerians with non-diabetic kidney disease [] |
