耐酒石酸酸性磷酸酶(ACP5)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Acid Phosphatase 5, Tartrate Resistant (ACP5)
TRAP; TrATPase; Tartrate Resistant Acid Phosphatase; Type 5 acid phosphatase
- 编号SEA902Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.312-20ng/mL
- 灵敏度最小可检测剂量小于等于0.129ng/mL.
- 样本类型Serum, plasma, tissue homogenates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2646 ¥ 3780 ¥ 17010 ¥ 32130 ¥ 264600
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测耐酒石酸酸性磷酸酶(ACP5)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的耐酒石酸酸性磷酸酶(ACP5)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 97-104 | 101 |
| EDTA plasma(n=5) | 89-96 | 92 |
| heparin plasma(n=5) | 78-95 | 83 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的耐酒石酸酸性磷酸酶(ACP5)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中耐酒石酸酸性磷酸酶(ACP5)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-98% | 80-92% | 78-104% | 89-103% |
| EDTA plasma(n=5) | 99-105% | 90-99% | 88-102% | 89-101% |
| heparin plasma(n=5) | 85-101% | 81-101% | 84-101% | 97-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将耐酒石酸酸性磷酸酶(ACP5)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的耐酒石酸酸性磷酸酶(ACP5)与连接于固相载体上的抗体结合，然后加入生物素化的耐酒石酸酸性磷酸酶(ACP5)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的耐酒石酸酸性磷酸酶(ACP5)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA902Hu01 | 耐酒石酸酸性磷酸酶(ACP5)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA902Hu01 | 耐酒石酸酸性磷酸酶(ACP5)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA902Hu22 | 耐酒石酸酸性磷酸酶(ACP5)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA902Hu | 耐酒石酸酸性磷酸酶(ACP5)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA902Hu | 耐酒石酸酸性磷酸酶(ACP5)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Archives of Oral Biology | Oxytocin promotes bone formation during the alveolar healing process in old acyclic female rats [ScienceDirect: S0003996912001021] |
| Life Sciences | Exendin-4 Increases Bone Mineral Density in Type 2 Diabetic OLETF Rats Potentially Through the Down-Regulation of SOST/Sclerostin in Osteocytes [PubMed: 23357248] |
| International Journal of Cardiology | Valvular osteoclasts in calcification and aortic valve stenosis severity. [Pubmed: 23452891] |
| Evidence-Based Complementary and Alternative Medicine | Antiosteoporosis Activity of New Oriental Medicine Preparation (Kyungokgo Mixed with Water Extract of Hovenia dulcis) on the Ovariectomized Mice [Pubmed:25737735] |
| Advances in Medical Sciences | The influence of bexarotene, a selective agonist of the retinoid receptor X (RXR), and tazarotene, a selective agonist of the retinoid acid receptor (RAR), on bone metabolism in rats [PubMed: 26569440] |
| Bone | The effects of strength training and raloxifene on bone health in aging ovariectomized rats [Pubmed:26812611] |
| Journal of Clinical Medicine Research | In Vitro and In Vivo Effects of Gracilaria verrucosa Extracts on Osteoclast Differentiation. [pubmed:28335442] |
| Biomedicine & Pharmacotherapy | Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats [S0753332216322752] |
| Clinical Trials in Degenerative Diseases | Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trial [issn:2542-3975] |
| Scientific Reports | Evidence for excessive osteoclast activation in SIRT6 null mice [Pubmed:30030453] |
| International Journal of Molecular Sciences | Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In Vivo [Pubmed:29562730] |
| Experimental Gerontology | The decline of whole-body glucose metabolism in ovariectomized rats [Pubmed: 30292771] |
| Bone Research | NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblasts [Pubmed: 30455992] |
| Cell Death & Disease | QKI deficiency leads to osteoporosis by promoting RANKL-induced osteoclastogenesis and disrupting bone metabolism [Pubmed: 32382069] |
| Effect of Corticopuncture (CP), Photobiomodulation (PBM) and the Combined Method on the Rate of Tooth Movement and Root Resorption: A Molecular, Histological … [] | |
| Evid Based Complement Alternat Med | Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway [34512780] |
