血管生成素1(ANGPT1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Angiopoietin 1 (ANGPT1)
ANG1; AGP1; AGPT; ANG-I
- 编号SEA008Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.24-15ng/mL
- 灵敏度最小可检测剂量小于等于0.11ng/mL.
- 样本类型Serum, plasma, tissue homogenates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2092 ¥ 2988 ¥ 13446 ¥ 25398 ¥ 209160
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测血管生成素1(ANGPT1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的血管生成素1(ANGPT1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 83-95 | 90 |
| EDTA plasma(n=5) | 86-94 | 90 |
| heparin plasma(n=5) | 81-94 | 88 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的血管生成素1(ANGPT1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管生成素1(ANGPT1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-92% | 82-102% | 78-103% | 83-97% |
| EDTA plasma(n=5) | 87-99% | 90-101% | 80-93% | 88-103% |
| heparin plasma(n=5) | 85-97% | 85-99% | 83-91% | 82-96% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将血管生成素1(ANGPT1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血管生成素1(ANGPT1)与连接于固相载体上的抗体结合，然后加入生物素化的血管生成素1(ANGPT1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管生成素1(ANGPT1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA008Hu01 | 血管生成素1(ANGPT1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA008Hu02 | 血管生成素1(ANGPT1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA008Hu03 | 血管生成素1(ANGPT1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA008Hu01 | 血管生成素1(ANGPT1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA008Hu02 | 血管生成素1(ANGPT1)多克隆抗体 | WB; IHC; ICC; IP. |
| FAA008Hu01 | 抗血管生成素1(ANGPT1)多克隆抗体 | Flow cytometry. |
| LAA008Hu71 | 血管生成素1(ANGPT1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA008Hu23 | 血管生成素1(ANGPT1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA008Hu24 | 血管生成素1(ANGPT1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA008Hu21 | 血管生成素1(ANGPT1)单克隆抗体 | WB |
| MAA008Hu22 | 血管生成素1(ANGPT1)单克隆抗体 | WB; IHC; ICC; IP. |
| FAA008Hu04 | 抗血管生成素1(ANGPT1)单克隆抗体 | Flow cytometry. |
| FAA008Hu02 | 抗血管生成素1(ANGPT1)单克隆抗体 | Flow cytometry. |
| FAA008Hu08 | 抗血管生成素1(ANGPT1)单克隆抗体 | Flow cytometry. |
| FAA008Hu06 | 抗血管生成素1(ANGPT1)单克隆抗体 | Flow cytometry. |
| LAA008Hu72 | 血管生成素1(ANGPT1)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA008Hu | 血管生成素1(ANGPT1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA008Hu | 血管生成素1(ANGPT1)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA008Hu | 血管生成素1(ANGPT1)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA008Hu | 血管生成素1(ANGPT1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Theriogenology | Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes [Science: Article] |
| J Atheroscler Thromb | High Mobility Group Box 1 Promotes Angiogenesis from Bone Marrow-derived Endothelial Progenitor Cells after Myocardial Infarction [PubMed: 25735431] |
| Archives of Biological Sciences | Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol [publication:299492265_Diabetes-induced_renal_failu] |
| Am J Transl Res | Angiopoietin-1 facilitates recovery of hematopoiesis in radiated mice [Pubmed:27347310] |
| Clinical research | Increased plasma concentrations of interleukin 35 in patients with coronary artery disease [Journal:-19] |
| Turkish Journal of Medicl Sciences | A new angiogenesis prognostic index with VEGFA, PlGF, and angiopoietin 1 predicts survival in patients with advanced gastric cancer [pubmed:28425270] |
| Sci Rep. | Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. [pubmed:28276491] |
| PeerJ | The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker [pubmed:28584715] |
| Molecular&cellular proteomics | Prediction of Response to Sorafenib in Hepatocellular Carcinoma: A Marker Panel by Multiple Reaction Monitoring-Mass Spectrometry [pubmed:28550167] |
| Tumor Biology | Cervical cancer cell–derived angiopoietins promote tumor progression [pubmed:28720059] |
| Free Patents online | RHEUMATOID ARTHRITIS RELATED BIOMARKER [20170350884] |
| Anesthesiology | Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … [Pubmed:28968277] |
| British Journal of Anaesthesia | Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats [10.1016:j.bja.2018.05.049] |
| The Journal of Biological Chemistry | Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice [Pubmed:29929978] |
| Clinical Significance of Nectin-4 Expression in Metastasis and Angiogenesis for Tumor Relapse [Doi: 10.4143/crt.2018.214] | |
| BIOLOGY OF REPRODUCTION | Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors [Pubmed: 30929911] |
| Scientific Reports | Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827] |
| Biochem Biophys Res Commun | Effects of interleukin-6 and tumor necrosis factor-α on the expression of angiogenic and collagenolytic factors in premature and mature adipocytes [Pubmed: 32800538] |
| J Nutr Biochem | Effects of protein malnutrition on haematopoietic regulatory activity of bone marrow mesenchymal stem cells [33705953] |
| Intensive Care Med Exp | The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats [33997943] |
| Gynecol Endocrinol | Potential of thrombospondin-1 in treatment of polycystic ovary syndrome rat model: a preliminary study [34282706] |
| Yonsei Med J | RAR-Related Orphan Receptor: An Accelerated Preeclampsia Progression by Activating the JAK/STAT3 Pathway [Pubmed:35619579] |
