二胺氧化酶(ABP1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Amiloride Binding Protein 1 (ABP1)
AOC1; DAO; KAO; Histaminase; Kidney amine oxidase; Diamine Oxidase; Amiloride-sensitive amine oxidase; Amine oxidase copper domain-containing protein 1
- 编号SEA656Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.312-20ng/mL
- 灵敏度最小可检测剂量小于等于0.124ng/mL.
- 样本类型serum, plasma, tissue homogenates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2646 ¥ 3780 ¥ 17010 ¥ 32130 ¥ 264600
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测二胺氧化酶(ABP1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的二胺氧化酶(ABP1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 84-105 | 90 |
| heparin plasma(n=5) | 80-101 | 91 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的二胺氧化酶(ABP1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中二胺氧化酶(ABP1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 99-105% | 78-88% | 78-102% | 94-102% |
| EDTA plasma(n=5) | 78-103% | 91-101% | 89-97% | 96-103% |
| heparin plasma(n=5) | 84-91% | 92-105% | 81-97% | 96-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将二胺氧化酶(ABP1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的二胺氧化酶(ABP1)与连接于固相载体上的抗体结合，然后加入生物素化的二胺氧化酶(ABP1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的二胺氧化酶(ABP1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA656Hu61 | 二胺氧化酶(ABP1)活性蛋白 | Cell culture; Activity Assays. |
| EPA656Hu61 | 二胺氧化酶(ABP1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA656Hu01 | 二胺氧化酶(ABP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA656Hu02 | 二胺氧化酶(ABP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA656Hu01 | 二胺氧化酶(ABP1)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| PAA656Hu02 | 二胺氧化酶(ABP1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA656Hu71 | 二胺氧化酶(ABP1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA656Hu22 | 二胺氧化酶(ABP1)单克隆抗体 | WB,IHC |
| MAA656Hu21 | 二胺氧化酶(ABP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA656Hu25 | 二胺氧化酶(ABP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA656Hu27 | 二胺氧化酶(ABP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA656Hu29 | 二胺氧化酶(ABP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA656Hu23 | 二胺氧化酶(ABP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA656Hu24 | 二胺氧化酶(ABP1)单克隆抗体 | WB; IHC; ICC; IP. |
| FAA656Hu02 | 抗二胺氧化酶(ABP1)单克隆抗体 | Flow cytometry. |
| FAA656Hu04 | 抗二胺氧化酶(ABP1)单克隆抗体 | FCM |
| FAA656Hu06 | 抗二胺氧化酶(ABP1)单克隆抗体 | FCM |
| FAA656Hu08 | 抗二胺氧化酶(ABP1)单克隆抗体 | FCM |
| SEA656Hu | 二胺氧化酶(ABP1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA656Hu | 二胺氧化酶(ABP1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Applied Clay Science | Effects of copper-exchanged montmorillonite, as alternative to antibiotic, on diarrhea, intestinal permeability and proinflammatory cytokine of weanling pigs [Cas.zju: Source] |
| Phytomedicine.? | A multi-component herbal preparation, STW 5, shows anti-apoptotic effects in radiation induced intestinal mucositis in rats [Pubmed:25022208] |
| Oxid Med Cell Longev | Elevation of HO-1 Expression Mitigates Intestinal Ischemia-Reperfusion Injury and Restores Tight Junction Function in a Rat Liver Transplantation Model [PubMed: 26064429] |
| Pancreatology. | Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis. [Pubmed:26804005] |
| Biochimica et Biophysica Acta (BBA)-General Subjects | Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats [pubmed:28341485] |
| Journal of Controlled Release | A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury [pubmed:28522195] |
| Advances in Experimental Medicine and Biology | Serum Diamine Oxidase in Pseudoallergy in the Pediatric Population [pubmed:28804811] |
| PLoS One | Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats [pubmed:28622357] |
| Nutrients | Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron [Pubmed:29861488] |
| Scientific Reports | Pregnancy-associated diamine oxidase originates from extravillous trophoblasts and is decreased in early-onset preeclampsia [Pubmed:29679053] |
| British Journal of Nutrition | Postoperative symbiotic in patients with head and neck cancer: a double-blind randomised trial [Pubmed:29277158] |
| Food & Function | The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer [Pubmed:29384543] |
| 硕士论文 | LOCALIZATION OF COPPER-CONTAINING AMINE OXIDASE ENZYMES IN THE BLOOD [] |
| International Journal of Clinical and Experimental Medicine | Effects of fecal microbiota transplantation and joint application of probiotics on rats with alcoholic liver disease [] |
| Journal of Functional Foods | Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease [] |
| Acta Diabetologica | Modulation of gut microbiota contributes to effects of intensive insulin therapy on intestinal morphological alteration in high-fat-diet-treated mice [Pubmed: 31749050] |
| Biomed Research International | Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet [Pubmed: 32149099] |
| FASEB JOURNAL | HMGB1‐associated necroptosis and Kupffer cells M1 polarization underlies remote liver injury induced by intestinal ischemia/reperfusion in rats [Pubmed: 31961020] |
| JOURNAL OF ETHNOPHARMACOLOGY | The effect of Poria cocos ethanol extract on the intestinal barrier function and intestinal microbiota in mice with breast cancer [Pubmed: 33039631] |
| Cell Death Discovery | Fecal microbiota transplantation from patients with autoimmune encephalitis modulates Th17 response and relevant behaviors in mice [Pubmed: 32821439] |
| The Journal of Nutritional Biochemistry | Effects of fucoidan on tumor prevention and gut flora [] |
| Chemical Biology & Dug Design | Design and development of novel fasudil derivatives as potent anti‐breast cancer agent that improves intestinal flora and intestinal barrier function in rats [34587363] |
| Journal of Functional Foods | Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways [] |
| Stem Cell Research & Therapy | Transplanted hair follicle mesenchymal stem cells alleviated small intestinal ischaemia–reperfusion injury via intrinsic and paracrine mechanisms in a rat model [] |
