纤连蛋白(FN)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Fibronectin (FN)
FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein
- 编号SEA037Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围1.56-100ng/mL
- 灵敏度最小可检测剂量小于等于0.69ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2008 ¥ 2868 ¥ 12906 ¥ 24378 ¥ 200760
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测纤连蛋白(FN)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的纤连蛋白(FN)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 80-105 | 91 |
| EDTA plasma(n=5) | 78-101 | 92 |
| heparin plasma(n=5) | 78-98 | 84 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的纤连蛋白(FN)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中纤连蛋白(FN)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 98-105% | 93-101% | 89-99% |
| EDTA plasma(n=5) | 95-103% | 92-101% | 83-93% | 96-104% |
| heparin plasma(n=5) | 85-101% | 79-103% | 95-104% | 78-101% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将纤连蛋白(FN)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的纤连蛋白(FN)与连接于固相载体上的抗体结合，然后加入生物素化的纤连蛋白(FN)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的纤连蛋白(FN)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA037Hu02 | 纤连蛋白(FN)活性蛋白 | Cell culture; Activity Assays. |
| RPA037Hu03 | 纤连蛋白(FN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA037Hu61 | 纤连蛋白(FN)活性蛋白 | Cell culture; Activity Assays. |
| EPA037Hu61 | 纤连蛋白(FN)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA037Hu01 | 纤连蛋白(FN)活性蛋白 | Cell culture; Activity Assays. |
| RPA037Hu01 | 纤连蛋白(FN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA037Hu02 | 纤连蛋白(FN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA037Hu01 | 纤连蛋白(FN)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| PAA037Hu04 | 纤连蛋白(FN)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA037Hu02 | 纤连蛋白(FN)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| PAA037Hu03 | 纤连蛋白(FN)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA037Hu71 | 纤连蛋白(FN)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA037Hu22 | 纤连蛋白(FN)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA037Hu | 纤连蛋白(FN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA037Hu | 纤连蛋白(FN)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA037Hu | 纤连蛋白(FN)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA037Hu | 纤连蛋白(FN)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA037Hu01 | 纤连蛋白(FN)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| American Journal of Physiology - Renal Physiology | Advanced oxidation protein products induce mesangial cell perturbation through PKC-dependent activation of NADPH oxidase [PubMed: 19019916] |
| Stem Cell Research Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly [Pubmed: 24739658] |
| Molecules. | Identification of compounds from the water soluble extract of Cinnamomum cassia barks and their inhibitory effects against high-glucose-induced mesangial cells. [Pubmed: 24013407] |
| Journal of Ethnopharmacology | Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathy [ScienceDirect: S0378874114002414] |
| Investigative Ophthalmology & Visual Science | Inhibition by Female Sex Hormones of Collagen Gel Contraction Mediated by Retinal Pigment Epithelial Cells [Pubmed: 24609629] |
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592] |
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747] |
| Laryngoscope. | Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria [Pubmed:25545625] |
| Biomedical Materials | In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study [Pubmed:25634132] |
| Biomedical Materials | In vivo [PubMed: 25634132] |
| American Journal of Medical and Biological Research | Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate onNF-κB/leptin-insulin Axisin Obese Subjects with Non-alcoholic Fatty Liver Disease [Ajmbr: 3] |
| Food Chem. | Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528] |
| journal of perinatal medicine | Maternal endothelial damage as a disorder shared by early preeclampsia, late preeclampsia and intrauterine growth restriction. [pubmed:27865093] |
| Chinese Journal of Contemporary Neurology and Neurosurgery | Effect of panax notoginseng saponins on efficacy and hemorrhagic transformation of rt-PA intravenous thrombolysis in patients with acute ischemic stroke [Article_en:CJFDTotal-XDJB201611014] |
| Scientific Reports | Serum fbronectin distinguishes the early stages of hepatocellular carcinoma [pubmed:28842594] |
| Molecular Medicine Reports | Pigment epithelium‑derived factor protects human glomerular mesangial cells from diabetes via NOXO1‑iNOS suppression [10.3892/mmr.2017.7563] |
| Cell Death & Disease | Tenomodulin is essential for prevention of adipocyte accumulation and fibrovascular scar formation during early tendon healing. [pubmed:29022912] |
| Journal of Cellular Biochemistry | Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL‐6 and IL‐8 in pulmonary fibrosis in rabbits [Pubmed:29932250] |
| Biomedicine & Pharmacotherapy | Corn silk (Zea mays L.), a source of natural antioxidants with α-amylase, α-glucosidase, advanced glycation and diabetic nephropathy inhibitory activities [Pubmed: 30530231] |
| Advanced Healthcare Materials | Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts [Pubmed: 30838809] |
| Inflammation | TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling [Pubmed: 31111299] |
| EBioMedicine | Three kinds of corneal host cells contribute differently to corneal neovascularization [] |
| Renal Failure | Effects of autophagy on macrophage adhesion and migration in diabetic nephropathy [Pubmed: 31352855] |
| Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy [Pubmed: 31511328] |
| Neurotherapeutics | Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair [Pubmed: 31758411] |
| FASEB Journal | Molecular hydrogen regulates PTEN‐AKT‐mTOR signaling via ROS to alleviate peritoneal dialysis‐related peritoneal fibrosis [Pubmed: 31930571] |
| Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109] |
| International Journal of Women¡¯s Health and Reproduction Sciences | Are the Circulating Levels of Copeptin and Fibronectin Dysregulated in Preeclamptic South African Black Women? [] |
| Advanced glycation end-products associate with podocytopathy in type II diabetic patients [] | |
| IBRO Neuroscience Reports | Early mobilization in spinal cord injury promotes changes in microglial dynamics and recovery of motor function [Pubmed:35586775] |
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