血红素氧合酶1(HO1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Heme Oxygenase 1 (HO1)
HMOX1; Hsp32; HMOX1D
- 编号SEA584Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.312-20ng/mL
- 灵敏度最小可检测剂量小于等于0.115ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2646 ¥ 3780 ¥ 17010 ¥ 32130 ¥ 264600
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测血红素氧合酶1(HO1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的血红素氧合酶1(HO1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 89-103 | 97 |
| EDTA plasma(n=5) | 87-101 | 97 |
| heparin plasma(n=5) | 85-101 | 89 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的血红素氧合酶1(HO1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血红素氧合酶1(HO1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-101% | 90-98% | 84-92% | 88-103% |
| EDTA plasma(n=5) | 78-98% | 89-99% | 93-101% | 98-105% |
| heparin plasma(n=5) | 81-94% | 91-103% | 94-101% | 90-98% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将血红素氧合酶1(HO1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血红素氧合酶1(HO1)与连接于固相载体上的抗体结合，然后加入生物素化的血红素氧合酶1(HO1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血红素氧合酶1(HO1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA584Hu01 | 血红素氧合酶1(HO1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA584Hu02 | 血红素氧合酶1(HO1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA584Hu01 | 血红素氧合酶1(HO1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA584Hu02 | 血红素氧合酶1(HO1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA584Hu71 | 血红素氧合酶1(HO1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA584Hu81 | 血红素氧合酶1(HO1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA584Hu21 | 血红素氧合酶1(HO1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA584Hu22 | 血红素氧合酶1(HO1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA584Hu | 血红素氧合酶1(HO1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA584Hu | 血红素氧合酶1(HO1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| The American Journal of Pathology | Characterization of the Intrarenal Renin-Angiotensin System in Experimental Alport Syndrome [PubMed: 25777063] |
| BioMed Research International | Serum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing Spondylitis [Pubmed:27314037] |
| Cancer Medicine | Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease [Pubmed:27109254] |
| Redox Report: Communications in Free Radical Research | Decreased expression of heme oxygenase is associated with depressive symptoms and may contribute to depressive and hypertensive comorbidity [Pubmed:26824276] |
| Canadian Journal of Physiology and Pharmacology | Sulforaphane protects against sodium valproate–induced acute liver injury [pubmed:28112972] |
| Peptides. | Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms. [pubmed:27777064] |
| european journal of pharmacology | S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase. [pubmed:27887948] |
| brain research | Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling. [pubmed:28238669] |
| biomedicine & pharmacotherapy | Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats. [pubmed:28068636] |
| Inflammopharmacology. | Implication of Nrf2/HO-1 pathway in the coloprotective effect of coenzyme Q10 against experimentally induced ulcerative colitis. [pubmed:28050757] |
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| Journal of Microbiology, Immunology and Infection | Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients [pubmed:28655573] |
| Nutrition & Metabolism | Hepatic, adipocyte, enteric and pancreatic hormones: response to dietary macronutrient composition and relationship with metabolism [pubmed:28694840] |
| Biomedicine & Pharmacotherapy | Ameliorative potential of sitagliptin and/or resveratrol on experimentallyinduced clear cell renal cell carcinoma [pubmed:29101811] |
| Acta Paediatrica | Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children [DOI: 10.1111/apa.14106] |
| Naunyn-Schmiedeberg's Arch Pharmacol | Quercetin and tin protoporphyrin attenuate hepatic ischemia reperfusion injury: role of HO-1 [pubmed:28589248] |
| Asian Pacific Journal of Cancer Prevention | Ameliorative Effect of Coenzyme Q10 and/or Candesartan on Carboplatin-Induced Nephrotoxicity: Roles of Apoptosis, Transforming Growth Factor-Β1, Nuclear Factor Kappa-B And The Nrf2/HO-1 Pathway [pubmed:28670881] |
| Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms [pubmed:28061403] |
| Talanta | Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment … [10.1016:j.talanta.2018.01.044] |
| Pharmacological Reports | The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder [10.1016:j.pharep.2018.06.001] |
| Physiology & Behavior | Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll … [Pubmed:29410018] |
| Journal of Infection and Chemotherapy | Targeting the proinflammatory cytokines, oxidative stress, apoptosis and TGF-β1/STAT-3 signaling by irbesartan to ameliorate doxorubicin-induced hepatotoxicity [Pubmed:29753615] |
| American Institute of Physics | Anti-hemolytic and anti-inflammatory activities of the methanolic extract of (P.Beauv.) Briq. leaves in 2-butoxyethanol-hemolytic induced … [10.1063:1.5033415] |
| Iranian Journal of Basic Medical Sciences | Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress [Doi: 10.22038/IJBMS.2018.31716.7714] |
| Biomedicine & Pharmacotherapy | Targeting Nrf2/HO-1 signaling by crocin: Role in attenuation of AA-induced ulcerative colitis in rats [Pubmed: 30530041] |
| Experimental Parasitology | Sodium arsenite augments sensitivity of Echinococcus granulosus protoscoleces to albendazole [Pubmed: 30790573] |
| JGH OPEN | Cilostazol against 2, 4, 6‐trinitrobenzene sulfonic acid‐induced colitis: Effect on tight junction, inflammation, and apoptosis [] |
| Journal of Food Biochemistry | Vanillic acid alleviates palmitic acid‐induced oxidative stress in human umbilical vein endothelial cells via Adenosine Monophosphate‐Activated Protein Kinase … [] |
| Experimental and Therapeutic Medicine | Effect of heme oxygenase 1 and renin/prorenin receptor on oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cells [] |
| Life Sciences | Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis [Pubmed: 31465731] |
| Respiratory physiology & neurobiology | Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosis [Pubmed: 31600583] |
| Life Sci | The protective effect of Lactobacillus versus 5-aminosalicylic acid in ulcerative colitis model by modulation of gut microbiota and Nrf2/Ho-1 pathway [Pubmed: 32526285] |
| JAMA Surgery | Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairment [Pubmed: 28593326] |
| JOURNAL OF PHARMACY AND PHARMACOLOGY | Isoliquiritigenin attenuates inflammation and modulates Nrf2/caspase-3 signalling in STZ-induced aortic injury [33793806] |
| Exp Parasitol | In vitro protoscolicidal effects of lithocholic acid on protoscoleces of Echinococcus granulosus and its mechanism [Pubmed:35594934] |
| Free Radical Biology and Medicine | Flavonoid 4, 4′-dimethoxychalcone induced ferroptosis in cancer cells by synergistically activating Keap1/Nrf2/HMOX1 pathway and inhibiting FECH [Pubmed:35697292] |
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