肝细胞生长因子(HGF)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Hepatocyte Growth Factor (HGF)
HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen
- 编号SEA047Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围31.2-2,000pg/mL
- 灵敏度最小可检测剂量小于等于11.5pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2008 ¥ 2868 ¥ 12906 ¥ 24378 ¥ 200760
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测肝细胞生长因子(HGF)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的肝细胞生长因子(HGF)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 87-94 | 91 |
| EDTA plasma(n=5) | 94-101 | 97 |
| heparin plasma(n=5) | 93-102 | 97 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的肝细胞生长因子(HGF)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中肝细胞生长因子(HGF)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-101% | 80-94% | 85-103% | 96-103% |
| EDTA plasma(n=5) | 78-97% | 94-103% | 80-90% | 83-94% |
| heparin plasma(n=5) | 90-103% | 96-103% | 87-103% | 91-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将肝细胞生长因子(HGF)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的肝细胞生长因子(HGF)与连接于固相载体上的抗体结合，然后加入生物素化的肝细胞生长因子(HGF)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的肝细胞生长因子(HGF)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA047Hu02 | 肝细胞生长因子(HGF)活性蛋白 | Cell culture; Activity Assays. |
| RPA047Hu03 | 肝细胞生长因子(HGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA047Hu01 | 肝细胞生长因子(HGF)活性蛋白 | Cell culture; Activity Assays. |
| RPA047Hu01 | 肝细胞生长因子(HGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA047Hu02 | 肝细胞生长因子(HGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA047Hu01 | 肝细胞生长因子(HGF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA047Hu02 | 肝细胞生长因子(HGF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA047Hu03 | 肝细胞生长因子(HGF)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA047Hu71 | 肝细胞生长因子(HGF)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA047Hu53 | 肝细胞生长因子(HGF)多克隆抗体(别藻蓝蛋白标记) | WB; IHC; ICC; IF. |
| LAA047Hu81 | 肝细胞生长因子(HGF)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA047Hu22 | 肝细胞生长因子(HGF)单克隆抗体 | WB,IHC |
| RAA047Hu21 | 肝细胞生长因子(HGF)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| MAA047Hu23 | 肝细胞生长因子(HGF)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA047Hu | 肝细胞生长因子(HGF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA047Hu | 肝细胞生长因子(HGF)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA047Hu | 肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA047Hu | 肝细胞生长因子(HGF)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA047Hu01 | 肝细胞生长因子(HGF)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
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| Journal of Translational Medicine | Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma [BioMed: 14795876] |
| Digestive Diseases and Sciences | Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver Failure [SpringerLink: k22823l138x2344x] |
| Biomarker insights | Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children [PubMed: PMC3694825] |
| Biomarker insights | Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604] |
| BMC cell biology | BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause [PubMed: PMC3640998] |
| Cell Journal (Yakhteh) | Effect of Mesenchymal Stem Cells on Doxorubicin-Induced Fibrosis [PubMed: PMC3584430] |
| Gastroenterol Res Pract | Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver Cirrhosis [PubMed: 26448742] |
| Tumor Biology | Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151] |
| Cytotherapy | Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injury [PubMed: 25863963] |
| Biomedicine & Pharmacotherapy | Silymarin-loaded Eudragit® RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated rats [Pubmed:27261582] |
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| Stem Cell Research & Therapy | Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats [10.1186/s13287-017-0739-3] |
| Oncology Letters | Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c‑Met/PI3K/AKT in vitro [10.3892/ol.2017.7301] |
| Stem Cell Research & Therapy | Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure. [pubmed:28679425] |
| Nature Communications | Pulmonary pericytes regulate lung morphogenesis [Pubmed:29934496] |
| Experimental And Molecular Medicine | Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke … [Pubmed:29650950] |
| Oncology Letters | Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro [Pubmed:29375714] |
| Bulletin of Experimental Biology and Medicine | Dynamics of Expression of Cytokine Genes and Macrophage Content in the Lungs and Kidneys after Subtotal Hepatectomy in Rats [Pubmed:29796817] |
| Cell Biology International | Inherent control of hepatocyte proliferationInherent control of hepatocyte proliferation after subtotal liver resection [Pubmed: 31297922] |
| Journal of stem cells & regenerative medicine | What is the Best Degree of Hyaluronic Acid Crosslinking in Increasing Growth Factors Level of Platelet-Rich Fibrin Lysate? [Pubmed: 31239605] |
| Open Journal of Veterinary Medicine | Efficacy of autologous bone marrow mononuclear cell transplantation in dogs with chronic spinal cord injury [Pubmed: 32821665] |
| Stem Cell Reviews and Reports | Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care [33469783] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … [Pubmed:35585377] |
