太阳集团tyc5997_平台(china)官方网站

色素上皮衍生因子(PEDF)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Pigment Epithelium Derived Factor (PEDF)

SERPINF1; EPC1; PIG35; SDF3; Serpin F1; Cell proliferation-inducing gene 35; Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin); Stromal Cell Derived Factor 3

  • 色素上皮衍生因子(PEDF)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 色素上皮衍生因子(PEDF)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 色素上皮衍生因子(PEDF)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEB972Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测色素上皮衍生因子(PEDF)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的色素上皮衍生因子(PEDF)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-90 82
EDTA plasma(n=5) 93-101 96
heparin plasma(n=5) 83-97 90

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的色素上皮衍生因子(PEDF)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中色素上皮衍生因子(PEDF)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 88-98% 79-98% 95-103% 92-103%
EDTA plasma(n=5) 86-99% 89-96% 85-103% 78-104%
heparin plasma(n=5) 96-104% 97-105% 78-104% 96-104%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将色素上皮衍生因子(PEDF)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的色素上皮衍生因子(PEDF)与连接于固相载体上的抗体结合，然后加入生物素化的色素上皮衍生因子(PEDF)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的色素上皮衍生因子(PEDF)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPB972Hu01 色素上皮衍生因子(PEDF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB972Hu01 色素上皮衍生因子(PEDF)多克隆抗体 WB; IHC; ICC; IP.
MAB972Hu22 色素上皮衍生因子(PEDF)单克隆抗体 WB; IHC; ICC; IP.
SEB972Hu 色素上皮衍生因子(PEDF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB972Hu 色素上皮衍生因子(PEDF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMB972Hu 色素上皮衍生因子(PEDF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB972Hu01 色素上皮衍生因子(PEDF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Molecular Therapy A Therapeutic Strategy for Choroidal Neovascularization Based on Recruitment of Mesenchymal Stem Cells to the Sites of Lesions [Nature: 2010144]
American Journal of Nephrology Urinary Pigment Epithelium-Derived Factor as a Marker of Diabetic Nephropathy [Karger: 314326]
34 Increased expression of pigment epithelium-derived factor in aged mesenchymal stem cells impairs their therapeutic efficacy for attenuating myocardial infarction injury [OxfordJournals: source]
Heart and Education in Heart The change of serum level of pigment epithelium-derived factor in coronary heart disease [Bmj: Source]
Mediators of Inflammation Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation [Pubmed: 24288442]
Cardiology. The association study of plasma levels of pigment epithelium-derived factor with acute coronary syndrome in the chinese han population. [Pubmed: 24192856]
Endocrinology Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent Mechanism [Endocrine: Source]
Clinical Science Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsis [PubMed: 25700221]
Molecular Sciences Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R [Pubmed:27187377]
Investigative ophthalmoloey & visual science The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal Pigment Epithelium Cells [articleid:2582822]
Scientific reports VEGF-B promotes recovery of corneal innervations and trophic functions in diabetic mice [pubmed:28091556]
国际眼科杂志 色素上皮衍生因子修饰的人脐带间充质干细胞构建方法 [201707007]
Investigative Ophthalmology & Visual Science PEDF Reduces the Severity of Herpetic Simplex Keratitis in Mice [Pubmed:30025136]
International Eye Science 高海拔地区新生血管性青光眼不同治疗方式的疗效及对房水中PEDF, VEGF 水平的影响 [2018/2/201802020.pdf]
Domestic Animal Endocrinology Lactation-related changes in tissue expression of PEDF in dairy cows [Pubmed:29758402]
Medical Science Monitor Correlation Between Pigment Epithelium-Derived Factor (PEDF) level and Degree of Coronary Angiography and Severity of Coronary Artery Disease in a … [Pubmed:29574467]
Chemosphere Role of pigment epithelium-derived factor (PEDF) on arsenic-induced neuronal apoptosis [Doi: 10.1016/j.chemosphere.2018.10.100]
Biological Trace Element Research Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat Model [Pubmed: 30392020]
International Urology and Nephrology Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement [Pubmed: 30536192]
International Journal of Ophthalmology A novel xeno-free culture system for human retinal pigment epithelium cells [Pubmed: 31024807]
Journal of the American Heart Association Pigment Epithelial‐Derived Factor Deficiency Accelerates Atherosclerosis Development via Promoting Endothelial Fatty Acid Uptake in Mice With Hyperlipidemia [Pubmed: 31711388]
environmental research Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA. hy926 cells [Pubmed: 32315827]
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells [33498962]
Hum Cell Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes ¡­ [33768511]
留言咨询
Baidu
sogou