类胰蛋白酶(TPS)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Tryptase (TPS)
TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III
- 编号SEB070Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围7.8-500pg/mL
- 灵敏度最小可检测剂量小于等于2.8pg/mL.
- 样本类型serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2793 ¥ 3990 ¥ 17955 ¥ 33915 ¥ 279300
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测类胰蛋白酶(TPS)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的类胰蛋白酶(TPS)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 92-99 | 96 |
| EDTA plasma(n=5) | 82-93 | 86 |
| heparin plasma(n=5) | 97-105 | 102 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的类胰蛋白酶(TPS)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中类胰蛋白酶(TPS)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 81-105% | 92-102% | 80-90% |
| EDTA plasma(n=5) | 91-103% | 87-101% | 85-103% | 80-101% |
| heparin plasma(n=5) | 85-103% | 87-96% | 90-104% | 98-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将类胰蛋白酶(TPS)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的类胰蛋白酶(TPS)与连接于固相载体上的抗体结合，然后加入生物素化的类胰蛋白酶(TPS)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的类胰蛋白酶(TPS)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB070Hu01 | 类胰蛋白酶(TPS)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB070Hu02 | 类胰蛋白酶(TPS)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB070Hu01 | 类胰蛋白酶(TPS)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB070Hu71 | 类胰蛋白酶(TPS)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB070Hu21 | 类胰蛋白酶(TPS)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB070Hu24 | 类胰蛋白酶(TPS)单克隆抗体 | WB,IHC |
| MAB070Hu26 | 类胰蛋白酶(TPS)单克隆抗体 | WB,IHC |
| MAB070Hu22 | 类胰蛋白酶(TPS)单克隆抗体 | WB; IHC |
| MAB070Hu23 | 类胰蛋白酶(TPS)单克隆抗体 | WB; IHC. |
| LAB070Hu76 | 类胰蛋白酶(TPS)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| FAB070Hu86 | 抗类胰蛋白酶(TPS)单克隆抗体(异硫氰酸荧光素标记) | FCM |
| WEB070Hu | 类胰蛋白酶(TPS)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB070Hu | 类胰蛋白酶(TPS)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB070Hu | 类胰蛋白酶(TPS)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB070Hu | 类胰蛋白酶(TPS)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSB070Hu01 | 类胰蛋白酶(TPS)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB070Hu01 | 类胰蛋白酶(TPS)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of Allergy and Clinical Immunology | Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps [PubMed: 22534535] |
| J Pediatr. | Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2 [Pubmed:24530123] |
| Mediators of Inflammation | Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis [Pubmed:25400334] |
| PLOS ONE | A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in Rats [Pubmed:25541997] |
| Neurogastroenterology & Motility | Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgery [Pubmed:25677271] |
| Mediators Inflamm | Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary Report [PubMed: 26089601] |
| Int Immunopharmacol | Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitis [PubMed: 26049029] |
| Mediators of Inflammation | Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell origins [Journals: Mi] |
| Mediators of Inflammation | Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell Origins [Pubmed:27057098] |
| Analytica Chimica Acta | An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis [] |
| Journal of Clinical Investigation | Dengue virus-elicited tryptase induces endothelial permeability and shock [Pubmed: 31265436] |
| Frontiers in Physiology | Mast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm Function [Pubmed: 31998139] |
| Biomarkers of Allergic Asthma and their Association with Serum Parameters [] | |
| Annals of Tropical Medicine & Public Health | Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity [] |
| Front Immunol | Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive … [34650557] |
| Microbiol Spectr | Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia Syndrome [Pubmed:35612327] |
