免疫球蛋白A(IgA)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Immunoglobulin A (IgA)
- 编号CEA546Ga
- 物种Chicken (Gallus，鸡) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围1.23-100ng/mL
- 灵敏度最小可检测剂量小于等于0.57ng/mL.
- 样本类型serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2982 ¥ 4260 ¥ 19170 ¥ 36210 ¥ 298200
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测免疫球蛋白A(IgA)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的免疫球蛋白A(IgA)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 88-101 | 94 |
| EDTA plasma(n=5) | 93-101 | 96 |
| heparin plasma(n=5) | 80-95 | 85 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的免疫球蛋白A(IgA)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中免疫球蛋白A(IgA)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-102% | 79-90% | 89-103% | 86-93% |
| EDTA plasma(n=5) | 92-101% | 99-105% | 85-98% | 81-101% |
| heparin plasma(n=5) | 81-101% | 88-102% | 83-101% | 95-102% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将免疫球蛋白A(IgA)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Chicken (Gallus，鸡) | 应用(仅供研究使用，不用于临床诊断！) |
| PAA546Ga01 | 免疫球蛋白A(IgA)多克隆抗体 | WB; IHC; ICC; IP. |
| SEA546Ga | 免疫球蛋白A(IgA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA546Ga | 免疫球蛋白A(IgA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA546Ga | 免疫球蛋白A(IgA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| IDOSI Publications | Sequence-Based Typing-Study on the Relationship Between Subclinical Mastitis and BoLA-DRB3.2* Allelic Polymorphism in Egyptian Cows [Idosi: Source] |
| international Journal of food and nutritional sciences | EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI [Ijfans: Source] |
| Poultry Science | Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 [Pubmed: 24570422 ] |
| Clinical and Vaccine Immunology | Specific humoral immune response induced by Propionibacterium acnes can prevent Actinobacillus pleuropneumoniae infection in mice [Asm: Source] |
| Clin Vaccine Immunol. | Speci?c Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in Mice [Pubmed:24429068] |
| Journal of Proteomics | Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection [Pubmed:24576640] |
| Food & Function | Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice [Pubmed:25308407] |
| Pediatrics | Oropharyngeal colostrum administration in extremely premature infants: an RCT. [Pubmed:25624376] |
| Front Microbiol | Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis [PubMed: 26217323] |
| Food and Agricultural Immunology | Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers [10.108:09540105.2017.1311308 ] |
| SYSTEMISCHE ENTZÜNDUNG BEI CHRONISCHER NIERENINSUFFIZIENZ – DIE ROLLE DER DARMFLORA [patent:US9763998B2] | |
| Water Research | Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model [Pubmed:29655095] |
| Vaccine | Recombinant Lactococcus lactis co-expressing OmpH of an M cell-targeting ligand and IBDV-VP2 protein provide immunological protection in chickens [Pubmed:29289381] |
| Food and Agricultural Immunology | Effects of and on growth performance, immune function and volatile fatty acid level of caecal digesta in broilers [10.1080:09540105.2018.1457013] |
| Probiotics Antimicrob Proteins | Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a … [Pubmed:29305686] |
| SAGE Open Medicine | Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetes [Pubmed:29623201] |
| Nephrology Dialysis Transplantation | The gut flora modulates intestinal barrier integrity but not progression of chronic kidney disease in hyperoxaluria-related nephrocalcinosis [Pubmed: 31081025] |
| Current Developments in Nutrition | Yacon (Smallanthus sonchifolius): Effect on Integrity of Intestinal Barrier, Inflammatory Response and Oxidative Stress in Animal Model of Colon Cancer (P06-052-19) [Pubmed: 31225040] |
| Nutrients. | Effects of Paramylon Extracted from Euglena gracilis EOD-1 on Parameters Related to Metabolic Syndrome in Diet-Induced Obese Mice [Pubmed: 31330894] |
| International Journal of Biological Macromolecules | Effects of polysaccharides from Yingshan Yunwu tea on meat quality, immune status and intestinal microflora in chickens [Pubmed: 32224178] |
| Effect Long-Term Exposure to Different Doses of Lipopolysaccharides on the Intestinal Mucosal Immune Barrier [] | |
| Italian Journal of Animal Science | Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the … [] |
| 博士论文 | Women Scientist Scientist Mentor [] |
| Acta Paediatr | MicroRNA profiling in Chinese children with Henoch©\Schonlein purpura and association between selected microRNAs and inflammatory biomarkers [33533510] |
| Environ Pollut | Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure [34090073] |
| J Ethnopharmacol | Monascus ruber fermented Panax ginseng ameliorates lipid metabolism disorders and modulate gut microbiota in rats fed a high-fat diet [34098018] |
| Microbiology Spectrum | Dietary Supplementation of Inulin Ameliorates Subclinical Mastitis via Regulation of Rumen Microbial Community and Metabolites in Dairy Cows [34494854] |
| Food Research International | Sulfated modification enhances the immunomodulatory effect of Cyclocarya paliurus polysaccharide on cyclophosphamide-induced immunosuppressed mice through … [34865774] |
| Food Funct | Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components [Pubmed:35438093] |
| Food and Agricultural Immunology | The effect of the multi-strain probiotic preparation EM Bokashi® on selected parameters of the cellular immune response in pigs [] |
| Journal of Functional Foods | Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways [] |
| Journal of Dentistr Journal of Dentistry Indonesia | Salivary IgA Depression in Drug-Influenced Gingival Enlargement among Hypertensive Patients [] |
