α1-酸性糖蛋白(a1AGP)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)
AGP1; a1-AGP; ORM1; OMD 1; Orosomucoid 1
- 编号SEA816Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围62.5-4,000ng/mL
- 灵敏度最小可检测剂量小于等于24.5ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2722 ¥ 3888 ¥ 17496 ¥ 33048 ¥ 272160
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测α1-酸性糖蛋白(a1AGP)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的α1-酸性糖蛋白(a1AGP)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 79-97 | 86 |
| EDTA plasma(n=5) | 78-99 | 80 |
| heparin plasma(n=5) | 95-105 | 98 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的α1-酸性糖蛋白(a1AGP)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中α1-酸性糖蛋白(a1AGP)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-104% | 91-99% | 78-101% | 96-105% |
| EDTA plasma(n=5) | 89-98% | 95-102% | 84-101% | 95-103% |
| heparin plasma(n=5) | 96-104% | 94-102% | 86-101% | 80-92% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将α1-酸性糖蛋白(a1AGP)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的α1-酸性糖蛋白(a1AGP)与连接于固相载体上的抗体结合，然后加入生物素化的α1-酸性糖蛋白(a1AGP)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的α1-酸性糖蛋白(a1AGP)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA816Mu01 | α1-酸性糖蛋白(a1AGP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA816Mu02 | α1-酸性糖蛋白(a1AGP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA816Mu01 | α1-酸性糖蛋白(a1AGP)多克隆抗体 | WB,IHC |
| PAA816Mu02 | α1-酸性糖蛋白(a1AGP)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA816Mu81 | α1-酸性糖蛋白(a1AGP)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA816Mu71 | α1-酸性糖蛋白(a1AGP)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA816Mu | α1-酸性糖蛋白(a1AGP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA816Mu | α1-酸性糖蛋白(a1AGP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA816Mu01 | α1-酸性糖蛋白(a1AGP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| J Reprod Dev.? | An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro [Pubmed:Pmc4219990] |
| Molecular Reproduction and Development | Evidence for a novel, local acute-phase response in the bovine oviduct: Progesterone and lipopolysaccharide up-regulate alpha 1-acid-glycoprotein expression in epithelial cells in vitro [Pubmed:25123565] |
| BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study [Pubmed:25652121] |
| PLoS One | Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study [PubMed: 25955845] |
| Sci Rep. | S100A9 and ORM1 serve as predictors of therapeutic response and prognostic factors in advanced extranodal NK/T cell lymphoma patients treated with pegaspargase/gemcitabine [pmc:PMC4810364] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
| Scientific Reports | An autoregressive logistic model to predict the reciprocal effects of oviductal fluid components on in vitro spermophagy by neutrophils in cattle [pubmed:28667317] |
| 33 | Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes. [pubmed:28927749] |
| Brazilian Journal of Otorhinolaryngology | Inflammatory markers in palatine tonsils of children with obstructive sleep apnea syndrome [Pubmed: 30213594] |
| BioRxiv | Proteome-wide characterization and biomarker identification of intracranial aneurysm [] |
| The FEBS journal | Dramatically changed immune‐related molecules as early diagnostic biomarkers of non‐small cell lung cancer [Pubmed: 31482685] |
| PLoS One | Effects of vaccination on acute-phase protein response in broiler chicken [Pubmed: 32191767] |
| Indian Journal of Animal Sciences | Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy … [] |
| A New Biomarker Tool for Risk Stratification in ¡°De Novo¡± Acute Heart Failure (OROME) [] |
