轴突生长诱向因子1(Ntn1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Netrin 1 (Ntn1)
NTN1L; Epididymis tissue protein Li 131P
- 编号SEB827Ga
- 物种Chicken (Gallus，鸡) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围15.62-1000pg/mL
- 灵敏度最小可检测剂量小于等于5.4pg/mL.
- 样本类型Serum, plasma and other biological fluids.
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2016 ¥ 2880 ¥ 12960 ¥ 24480 ¥ 201600
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测轴突生长诱向因子1(Ntn1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的轴突生长诱向因子1(Ntn1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 80-94 | 83 |
| EDTA plasma(n=5) | 91-102 | 97 |
| heparin plasma(n=5) | 81-99 | 87 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的轴突生长诱向因子1(Ntn1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中轴突生长诱向因子1(Ntn1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-105% | 99-105% | 96-105% | 98-105% |
| EDTA plasma(n=5) | 93-101% | 88-105% | 96-105% | 78-92% |
| heparin plasma(n=5) | 92-104% | 89-97% | 79-101% | 88-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将轴突生长诱向因子1(Ntn1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的轴突生长诱向因子1(Ntn1)与连接于固相载体上的抗体结合，然后加入生物素化的轴突生长诱向因子1(Ntn1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的轴突生长诱向因子1(Ntn1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Chicken (Gallus，鸡) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB827Ga01 | 轴突生长诱向因子1(Ntn1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB827Ga01 | 轴突生长诱向因子1(Ntn1)多克隆抗体 | WB; IHC; ICC; IP. |
| MAB827Ga21 | 轴突生长诱向因子1(Ntn1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB827Ga | 轴突生长诱向因子1(Ntn1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB827Ga | 轴突生长诱向因子1(Ntn1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Transplantation Proceedings | Netrin-1: A Novel Universal Biomarker of Human Kidney Injury [PubMed: 20620466] |
| Biomarkers | Plasma netrin-1 is a diagnostic biomarker of human cancers [PubMed: PMC3143477] |
| Journal of Maternal-Fetal and Neonatal Medicine | Cord blood netrin-1 and -4 concentrations in term pregnancies with normal, restricted and increased fetal growth [Informahealthcare: Source] |
| 4 | Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity [Pubmed:24584118] |
| J Matern?Fetal?Neonatal Med.? | Cord blood netrin-1 and-4 concentrations in term pregnancies with normal, restricted and increased fetal growth [Pubmed:24716747] |
| Renal failure. | Urinary netrin-1 and KIM-1 as early biomarkers for septic acute kidney injury [Pubmed:25154466] |
| Stem Cell Research & Therapy | Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton’s jelly mesenchymal stem cells (WJ-MSC) [articles:10.1186] |
| Oncotarget | The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium. [pubmed:29156815] |
| Frontiers in Cellular Neuroscience | Neuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in Rats [Pubmed:29375318] |
| Scientific Reports | Netrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor [Pubmed:29662125] |
| Family Practice and Palliative Care | Relationship of serum netrin-1 levels with breast masses [Doi: 10.22391/fppc.467700] |
| JOURNAL OF BIOLOGICAL CHEMISTRY | Role of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesity [Pubmed: 30910812] |
| International Journal of Molecular Sciences | Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus [Pubmed: 30897795] |
| Cancer Biomark | Serum netrin-1 as a biomarker for colorectal cancer detection [Pubmed: 32474463] |
| Níveis Elevados de Netrina-1 e IL-1β em Mulheres Idosas com SCA: Pior Prognóstico no Acompanhamento de Dois Anos [] | |
| Tuberk Toraks | Association of plasma netrin-1 level with inflammatory balance and comorbidities in patients with acute exacerbation of COPD [33853303] |
| Adv Healthc Mater | Conductive Hydrogel Conduits with Growth Factor Gradients for Peripheral Nerve Repair in Diabetics with Non‐suture Tape [Pubmed:35670309] |
