神经肽Y(NPY)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Neuropeptide Y (NPY)
NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY
- 编号CEA879Gu
- 物种Cavia (Guinea pig ，豚鼠) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围24.69-2,000pg/mL
- 灵敏度最小可检测剂量小于等于8.87pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3024 ¥ 4320 ¥ 19440 ¥ 36720 ¥ 302400
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测神经肽Y(NPY)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的神经肽Y(NPY)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 94-103 | 98 |
| EDTA plasma(n=5) | 94-105 | 97 |
| heparin plasma(n=5) | 90-101 | 95 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的神经肽Y(NPY)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中神经肽Y(NPY)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-97% | 94-102% | 82-91% | 86-99% |
| EDTA plasma(n=5) | 98-105% | 94-101% | 78-101% | 85-102% |
| heparin plasma(n=5) | 79-89% | 78-103% | 93-102% | 92-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将神经肽Y(NPY)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Cavia (Guinea pig ，豚鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| CEA879Gu | 神经肽Y(NPY)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA879Gu | 神经肽Y(NPY)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Hemodialysis International | Is there any interaction of resistin and adiponectin levels with protein‐energy wasting among patients with chronic kidney disease [Onlinelibrary: hdi.12072] |
| Journal of Pediatric Endocrinology and Metabolism | Changes in growth pattern, leptin ghrelin and neuropeptide Y levels after adenotonsillectomy in prepubertal children [Degruyter: Source] |
| Ren Fail. | The role of adipocytokines on depressive symptoms of patients with chronic kidney disease [Pubmed: 23879728] |
| Coronary Artery Disease | The relationship of plasma neuropeptide Y levels with coronary collateral development. [Pubmed: 24077326] |
| Coronary Artery Disease | The relationship of plasma neuropeptide Y levels with coronary collateral development [Lww:Source] |
| Toxicology Letters | Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate [Pubmed:25623392] |
| J Neurosurg Anesthesiol | High Plasma Levels of Neuropeptide Y Correlate With Good Clinical Outcome But are not Correlated to Cerebral Blood Flow or Vasospasm After Subarachnoid Hemorrhage [PubMed: 25955865] |
| Journal of Diabetes Research | High Intensity Aerobic Exercise Training Improves Deficits of Cardiovascular Autonomic Function in a Rat Model of Type 1 Diabetes Mellitus with Moderate … [Pubmed:26885531] |
| Archives of Gynecology and Obstetrics | Binge eating and biochemical markers of appetite in new users of the contraceptive depot medroxyprogesterone acetate [pubmed:27604241] |
| basic & clinical pharmacology & toxicology | Effects of Di-isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells. [pubmed:27625336] |
| Acta Neurobiologiae Experimentalis | Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat [pubmed:27685769] |
| Cardiovascular research | Nur77 protects against adverse cardiac remodelling by limiting Neuropeptide Y signalling in the sympathoadrenal-cardiac axis [Pubmed:29850786] |
| Journal of Periodontology | Salivary neuropeptides, stress, and periodontitis [Pubmed:28914594] |
| Neuropeptides | Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor [Pubmed:29685637] |
| Pharmacology | Melatonin Induces Osteoblastic Differentiation of Mesenchymal Stem Cells and Promotes Fracture Healing in a Rat Model of Femoral Fracture via Neuropeptide Y … [Pubmed: 30227410] |
| Marmara Medical Journal | Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome [] |
| Marmara Medical Journal | Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome. [] |
| neurochemical research | Sevoflurane Induces Hippocampal Neuronal Apoptosis by Altering the Level of Neuropeptide Y in Neonatal Rats. [Pubmed: 32378074] |
| Hum Psychopharmacol | Serum levels of neuropeptide Y in patients with chronic schizophrenia during treatment augmentation with sarcosine (results of the double©\blind randomized?¡ [33245168] |
| Arch Toxicol | Serotonin involvement in okadaic acid-induced diarrhoea in vivo [34148100] |
