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载脂蛋白E(APOE)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Apolipoprotein E (APOE)

Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset

  • 载脂蛋白E(APOE)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 载脂蛋白E(APOE)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 载脂蛋白E(APOE)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA704Po.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测载脂蛋白E(APOE),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的载脂蛋白E(APOE)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 81-102 98
EDTA plasma(n=5) 96-105 101
heparin plasma(n=5) 92-103 95

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的载脂蛋白E(APOE),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中载脂蛋白E(APOE)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 91-103% 79-102% 92-101%
EDTA plasma(n=5) 80-93% 80-98% 84-104% 88-96%
heparin plasma(n=5) 94-101% 78-98% 89-97% 80-92%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将载脂蛋白E(APOE)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的载脂蛋白E(APOE)与连接于固相载体上的抗体结合,然后加入生物素化的载脂蛋白E(APOE)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的载脂蛋白E(APOE)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Sus scrofa; Porcine (Pig,猪) 应用(仅供研究使用,不用于临床诊断!)
RPA704Po01 载脂蛋白E(APOE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA704Po01 载脂蛋白E(APOE)多克隆抗体 WB; IHC; ICC; IP.
MAA704Po21 载脂蛋白E(APOE)单克隆抗体 WB; IHC; ICC; IP.
SEA704Po 载脂蛋白E(APOE)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA704Po 载脂蛋白E(APOE)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA704Po01 载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It(ELISA Kit) Yourself"

参考文献

杂志 参考文献
Seizure - European Journal of Epilepsy Cerebrospinal fluid apolipoprotein E concentration decreases after seizure [Seizure: PIIS1059131109002441]
Biochimica et Biophysica Acta Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties [PubMed: 23328279 ]
Medical Oncology Expression and clinical significance of apolipoprotein E in pancreatic ductal adenocarcinoma [Pubmed: 23609192]
Diabetes. Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity [Pubmed:25249572]
renal failure Frequencies of apolipoprotein E alleles in depressed patients undergoing hemodialysis – a case-control study [PubMed: 25707516]
Biochim Biophys Acta Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253]
Diabetes Adipocyte ATP-binding cassette G1 promotes triglyceride storage, fat mass growth, and human obesity [PubMed: 25249572]
Scientific Reports Adaptive immunity against gut microbiota enhances apoE-mediated immune regulation andreduces atherosclerosis and western-diet-related inflammation. [pubmed:27383250]
Biochemical Pharmacology  Differential effects of apoE and apoJ mimetic peptides on the action of an anti-Aβ scFv in 3xTg-AD mice [Pubmed:30026023]
Oncotarget Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse … [Pubmed:29731975]
Proteomics Clinical Applications Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy [Pubmed: 31162828]
Nature Communications Hepatic HuR modulates lipid homeostasis in response to high-fat diet [Pubmed: 32546794]
VETERINARY PATHOLOGY Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats [Pubmed: 32880234]
Journal of Clinical Medicine The Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome [33321701]
Cancer Cell Int Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer [33602229]
Nat Commun GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313]
Talanta Iridium nanoclusters as high sensitive-tunable elemental labels for immunoassays: Determination of IgE and APOE in aqueous humor by inductively coupled … [Pubmed:35378356]
Analytica Chimica Acta Gold nanoclusters as elemental label for the sequential quantification of apolipoprotein E and metallothionein 2A in individual human cells of the retinal … [Pubmed:35361429]
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